Centers & Labs

RIKEN Center for Sustainable Resource Science

Molecular Structure Characterization Unit

Unit Leader: Hiroyuki Koshino (D.Agr.)
Hiroyuki  Koshino(D.Agr.)

We develop new methods and technologies of NMR and MS analyses for structural elucidation and characterization of novel organic compounds that are found or synthesized in chemistry and related scientific fields such as chemical biology, metabolomics research, and several organic synthetic studies. We provide diverse research support activity for characterization of organic molecules through maintenance and operation of MS, NMR, and CD facilities for all RIKEN researchers. Our research supporting activities include training on open access machines, technical assistance, data acquisition, and spectral data analysis and interpretation. We collaborate with many research groups, and continue to improve our capability and methodology for organic molecular characterization and structural determination by spectroscopic analysis together with organic synthesis.

Main Research Field

Chemistry

Related Research Fields

Interdisciplinary science and engineering / Agricultural sciences / Medicine, dentistry, and pharmacy

Keywords

  • Instrumental analysis
  • NMR
  • Structural elucidation
  • Mass spectrometry
  • Chemical biology

Selected Publications

  1. Furuhashi, T., Ishii, K., Tanaka, K., Weckwerth, W., and Nakamura, T.:
    "Fragmentation patterns of methyloxime-trimethylsilyl derivatives of constitutive mono- and disaccharide isomers analyzed by gas chromatography/field ionization mass spectrometry"
    Rapid Commun. Mass Spectrom. 29, 238 (2015).
  2. Nakamura, T., Tamada, D., Yanagi, Y., Itoh, Y., Nemoto, T., Utsumi, H., and Kose, K.:
    "Development of a superconducting bulk magnet for NMR and MRI"
    J. Magn. Reson. 295, 68 (2015).
  3. Nishikawa, Y., Furukawa, A., Shiga, I., Muroi, Y., Ishii, T., Hongo, Y., Takahashi, S., Sugawara, T., Koshino, H., and Ohnishi, M.:
    "Cytoprotective effects of lysophospholipids from sea cucumber Holothuria atra"
    PLoS ONE 10, e0135701 (2015).
  4. Mukhina, O. A., Koshino, H., Crimmins, M. T., and Kutateladze, A. G.:
    "Computationally driven reassignment of the structures of aldingenins A and B"
    Tetrahedron Lett. 56, 4900 (2015).
  5. Hongo, Y., Nakamura, T., Takahashi, S., Motoyama, T., Hayashi, T., Hirota, H., Osada, H., and Koshino, H.:
    "Detection of oxygen addition peaks for terpendole E and related indole-diterpene alkaloids in a positive-mode ESI-MS"
    J. Mass Spectrom. 49, 537 (2014).
  6. Koichi, S., Arisaka, M., Koshino, H., Aoki, A., Iwata, S., Uno, T., and Satoh, H.:
    "Chemical structure elucidation from 13C NMR chemical shifts: efficient data processing using bipartite matching and maximal clique algorithms"
    J. Chem. Inf. Model. 54, 1027 (2014).
  7. Takahashi, S., Yasuda, M., Nakamura, T., Hatano, K., Matsuoka, K., and Koshino, H.:
    "Synthesis and structural revision of a brominated sesquiterpenoid, aldingenin C"
    J. Org. Chem. 79, 9373 (2014).
  8. Takahashi, S., Satoh, D., Hayashi, M., Takahashi, K., Yamaguchi, K., Nakamura, T., and Koshino, H.:
    "Totall synthesis of the proposed structure for aromin and its structural revision"
    J. Org. Chem., 81, 11222 (2016).
  9. Sun, X., Hirai, G., Ueki, M., Hirota, H., Wang, Q., Hongo, Y., Nakamura, T., Hirota, Y., Takahashi, H., Sodeoka, M., Osada, H., Hamamoto, M., Yoshida, M., and Yashiroda, Y.:
    "Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast"
    Sci. Rep. 6, 20856 (2016).
  10. Kusakabe, K., Honmura, Y., Uesugi, S., Tonouchi, A., Maeda, H., Kimura, K., Koshino, H.:
    "Neomacrophorin X, a [4.4.3]propellane-type meroterpenoid from Trichoderma sp. 1212-03"
    J. Nat. Prod., 80, 1484 (2017).

Lab Members

Principal Investigator

Hiroyuki Koshino
Unit Leader

Core Members

Shun-ya Takahashi
Senior Research Scientist
Takemichi Nakamura
Senior Research Scientist
Takashi Nakamura
Senior Technical Scientist

Contact information

308 Chemical Biology Bldg.,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan
Tel: +81-(0)48-467-9361
Fax: +81-(0)48-462-1640

Email: koshino [at] riken.jp

Related links