Centers & Labs

RIKEN Center for Integrative Medical Sciences

Laboratory for Developmental Genetics

Group Director: Haruhiko Koseki (M.D., Ph.D)
Haruhiko  Koseki(M.D., Ph.D)

The Developmental Genetics Research Group is pursuing a research program to elucidate the epigenetic regulation of organ development and stem cell functions by Polycomb group (PcG) proteins and DNA methylation mechanisms. Our group is also responsible to operate the animal facility affiliated to Yokohama Institute that includes cryopreservation and generation of genetic resources and human iPS cell facility.

Main Research Field

Molecular Biology & Genetics

Related Research Fields

Biology & Biochemistry / Immunology

Research Subjects

  • Regulation of large scale chromatin structures by Polycomb
  • Chromatin dynamics associated with activation of Polycomb-repressed genes
  • Development for anti-tumor immunotherapy by using iPSC-derived NKT cells

Selected Publications

  1. Kondo T, Ito S, Koseki H.:
    “Polycomb in Transcriptional Phase Transition of Developmental Genes.”
    Trends Biochem Sci 41, 9-19 (2016).
  2. Yakushiji-Kaminatsui N, Kondo T, Endo TA, Koseki Y, Kondo K, Ohara O, Vidal M, Koseki H.:
    “RING1 proteins contribute to early proximal-distal specification of the forelimb bud by restricting Meis2 expression.”
    Development 143, 276-85 (2016).
  3. Obata Y, Furusawa Y, Endo TA, Sharif J, Takahashi D, Atarashi K, Nakayama M, Onawa S, Fujimura Y, Takahashi M, Ikawa T, Otsubo T, Kawamura YI, Dohi T, Tajima S, Masumoto H, Ohara O, Honda K, Hori S, Ohno H, Koseki H, Hase K.:
    "The epigenetic regulator Uhrf1 facilitates the proliferation and maturation of colonic regulatory T cells."
    Nat Immunol 15, 571-579 (2014).
  4. Blackledge NP, Farcas AM, Kondo T, King HW, McGouran JF, Hanssen LL, Ito S, Cooper S, Kondo K, Koseki Y, Ishikura T, Long HK, Sheahan TW, Brockdorff N, Kessler BM, Koseki H, Klose RJ.:
    "Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation."
    Cell 157, 1445-1459 (2014).
  5. Benitah SA, Bracken A, Dou Y, Huangfu D, Ivanova N, Koseki H, Laurent L, Lim DA, Meshorer E, Pombo A, Sander M, Xu GL.:
    "Stem cell epigenetics: looking forward."
    Cell Stem Cell 14, 706-709 (2014).
  6. Kondo T., Isono K, Kondo K, Endo TA, Itohara S, Vidal M, and Koseki H.:
    "The Polycomb Potentiates Meis2 Activation in Midbrain by Mediating Interaction of the Promoter with a Tissue-Specific Enhancer."
    Dev Cell 28, 94-101 (2014).
  7. Isono K, Endo TA, Ku M, Yamada D, Suzuki R, Sharif J, Ishikura T, Toyoda T, Bernstein BE, Koseki H.:
    "SAM Domain Polymerization Links Subnuclear Clustering of PRC1 to Gene Silencing."
    Dev Cell 26, 565-577 (2013).
  8. Sharif J, Endo TA, Ito S, Ohara O, Koseki H.:
    "Embracing change to remain the same: conservation of polycomb functions despite divergence of binding motifs among species."
    Curr Opin Cell Biol 25, 305-313 (2013).
  9. Sharif J, Shinkai Y, Koseki H.:
    "Is there a role for endogenous retroviruses to mediate long-term adaptive phenotypic response upon environmental inputs?"
    Philos Trans R Soc Lond B Biol Sci 368, 20110340 (2013).
  10. Vizcardo R, Masuda K, Yamada D, Ikawa T, Shimizu K, Fujii S, Koseki H, Kawamoto H.:
    "Regeneration of Human Tumor Antigen-Specific T Cells from iPSCs Derived from Mature CD8(+) T Cells."
    Cell Stem Cell 12, 31-36 (2013).

Lab Members

Principal Investigator

Haruhiko Koseki
Group Director

Core Members

Kyoichi Isono
Senior Research Scientist
Takanori Hasegawa
Senior Technical Scientist
Yu-ichi Fujimura
Research Scientist
Yixin Dong
Research Scientist
Daisuke Yamada
Research Scientist
Jafar Sharif
Research Scientist
Osamu Masui
Research Scientist
Shinsuke Ito
Research Scientist
Kit Wan Ma
Research Scientist
Shinobu Mochizuki
Technical Scientist
Masashi Matsuda
Technical Scientist
Satoshi Oonawa
Technical Scientist
Juan Guillermo Betancur Medina
Special Postdoctoral Researcher
Rumi Sato
Research Associate
Junichiro Takano
Junior Research Associate
Midori Kobayashi
Technical Staff I
Tomoyuki Ishikura
Technical Staff I
Chieko Tezuka
Technical Staff I
Takako Yamamoto
Technical Staff I
Momoko Okoshi
Technical Staff I
Genta Kitahara
Technical Staff I
Yusuke Iizuka
Technical Staff I
Hiromi Tatsumi
Technical Staff I
Yurie Kawamoto
Technical Staff I
Kayoko Katsuyama
Technical Staff II
Yoko Koseki
Technical Staff II
Fuyuko Shiotani
Technical Staff II
Mami Kumon
Technical Staff II
Miho Urabe
Technical Staff II
Marika Shibata
Technical Staff II

Contact information

North Research Building,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan

Email: haruhiko.koseki [at]

Related links

Recent research results

July 18, 2014: RIKEN RESEARCH
Gene regulation turned upside down