Centers & Labs

RIKEN Center for Integrative Medical Sciences

Laboratory for Metabolomics

Team Leader: Makoto Arita (Ph.D.)
Makoto  Arita(Ph.D.)

Our research is aimed at elucidating structure and function of endogenous lipid mediators that regulate inflammation and tissue homeostasis. LC-MS/MS-based lipidomics concerns the simultaneous and quantitative analysis to find a potential link between lipid metabolism and biological phenotype. Our current research is focused on understanding ω3 PUFA’s function using genetic and lipidomic approaches, and has identified novel metabolic pathway and bioactive metabolites that may link to ω3 PUFA’s anti-inflammatory actions. These endogenous mediators with potent anti-inflammatory property could lead to the development of novel therapeutics for disease when sustained inflammation is suspected as key components of pathogenesis. Also we put a lot of efforts into development and application of advanced LC-MS/MS-based lipidomics system to discover novel bioactive metabolites.

Main Research Field

Medicine, dentistry, and pharmacy

Related Research Fields

Chemistry / Biological Sciences / Biology

Keywords

  • Lipidomics
  • Bioactive Lipid
  • Regulation of Inflammation
  • Fatty Acid

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.
  1. Aoyagi R, Ikeda K, Isobe Y, Arita M.
    "Comprehensive analyses of oxidized phospholipids using a measured MS/MS spectra library."
    J Lipid Res in press (2017)
  2. Tsugawa H, Ikeda K, Arita M.
    "The importance of bioinformatics for connecting data-driven lipidomics and biological insights."
    Biochim Biophys Acta 1862, 762-765 (2017)
  3. Hirabayashi T, Anjo T, Kaneko A, Senoo Y, Shibata A, Takama H, Yokoyama K, Nishito Y, Ono T, Taya C, Muramatsu K, Fukami K, Muñoz-Garcia A, Brash AR, Ikeda K, Arita M, Akiyama M, Murakami M.
    "PNPLA1 has a crucial role in skin barrier function by directing acylceramide biosynthesis."
    Nat Commun 8, 14609 (2017)
  4. Loo TM, Kamachi F, Watanabe Y, Yoshimoto S, Kanda H, Arai Y, Nakajima-Takagi Y, Iwama A, Koga T, Sugimoto Y, Ozawa T, Nakamura M, Kumagai M, Watashi K, Taketo M, Aoki T, Narumiya S, Oshima M, Arita M, Hara E, Ohtani N.
    "Gut microbiota promotes obesity-associated liver cancer through PGE2-mediated suppression of antitumor immunity."
    Cancer Discov 7, 522-538 (2017)
  5. Sakayori N, Kikkawa T, Tokuda H, Kiryu E, Yoshizaki K, Kawashima H, Yamada T, Arai H, Kang JX, Katagiri H, Shibata H, Innis SM, Arita M, Osumi N.
    "Maternal dietary balance between omega-6 and omega-3 polyunsaturated fatty acids impairs neocortical development via epoxy metabolites."
    Stem Cells 34, 470-482 (2016)
  6. Kunisawa J, Arita M, Hayasaka T, Harada T, Iwamoto R, Nagasawa R, Shikata S, Nagatake T, Suzuki H, Hashimoto E, Kurashima Y, Suzuki Y, Arai H, Setou M, Kiyono H.
    "Dietary ω3 fatty acid exerts anti-allergic effect through the conversion to 17,18-epoxyeicostetraenoic acid in the gut."
    Sci Rep 5, 9750 (2015)
  7. Endo J, Sano M, Isobe Y, Fukuda K, Kang JX, Arai H, Arita M.
    "18-HEPE, an n-3 fatty acid metabolite released by macrophages, prevents pressure overload-induced maladaptive cardiac remodeling."
    J Exp Med 211, 1673-1687 (2014)
  8. Tani Y, Isobe Y, Imoto Y, Segi-Nishida E, Sugimoto Y, Arai H, Arita M.
    "Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice."
    FASEB J 28, 4036-4043 (2014)
  9. Kubota T, Arita M, Isobe Y, Iwamoto R, Goto T, Yoshioka T, Urabe D, Inoue M, Arai H.
    "Eicosapentaenoic acid is converted via omega-3 epoxygenation to anti-inflammatory metabolite 12-hydroxy-17,18-epoxyeicosatetraenoic acid."
    FASEB J 28, 586-593 (2014)
  10. *Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanabe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, Imai Y.
    "The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza."
    Cell 153, 112-125 (2013)

Lab Members

Principal Investigator

Makoto Arita
Team Leader

Core Members

Ken-ichi Moto
Senior Research Scientist
Kazutaka Ikeda
Senior Research Scientist
Tomoaki Ishihara
Research Scientist
Yosuke Isobe
Special Postdoctoral Researcher
Tatsuro Naganuma
Special Postdoctoral Researcher
Ryohei Aoyagi
Junior Research Associate
Mamoru Ogawa
Junior Research Associate
Mie Honda
Technical Staff I
Yuuya Senoo
Technical Staff I
Miwa Yoshimoto
Technical Staff I

Contact information

North Research Building,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Tel: +81-(0)45-503-7055
Fax: +81-(0)45-503-7054

Email: makoto.arita [at] riken.jp

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