Centers & Labs

RIKEN Center for Integrative Medical Sciences

Laboratory for Metabolomics

Team Leader: Makoto Arita (Ph.D.)
Makoto  Arita(Ph.D.)

Our research is aimed at elucidating structure and function of endogenous lipid mediators that regulate inflammation and tissue homeostasis. LC-MS/MS-based lipidomics concerns the simultaneous and quantitative analysis to find a potential link between lipid metabolism and biological phenotype. Our current research is focused on understanding ω3 PUFA’s function using genetic and lipidomic approaches, and has identified novel metabolic pathway and bioactive metabolites that may link to ω3 PUFA’s anti-inflammatory actions. These endogenous mediators with potent anti-inflammatory property could lead to the development of novel therapeutics for disease when sustained inflammation is suspected as key components of pathogenesis. Also we put a lot of efforts into development and application of advanced LC-MS/MS-based lipidomics system to discover novel bioactive metabolites.

Main Research Field

Biology & Biochemistry

Related Research Fields

Chemistry / Molecular Biology & Genetics / Immunology / Pharmacology & Toxicology / Multidisciplinary

Research Subjects

  • Genetic and lipidomic approach to understand omega-3 polyunsaturated fatty acid’s anti-inflammatory properties
  • Cellular and molecular mechanisms underlying the resolution of inflammation
  • Development and application of advanced LC-MS/MS-based lipidomic analyses

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.
  1. Endo J, Sano M, Isobe Y, Fukuda K, Kang JX, Arai H, Arita M.:
    “18-HEPE, an n-3 fatty acid metabolite released by macrophages, prevents pressure overload-induced maladaptive cardiac remodeling.”
    J Exp Med 211, 1673-1687 (2014)
  2. Tani Y, Isobe Y, Imoto Y, Segi-Nishida E, Sugimoto Y, Arai H, Arita M.:
    “Eosinophils control the resolution of inflammation and draining lymph node hypertrophy through the proresolving mediators and CXCL13 pathway in mice.”
    FASEB J 28, 4036-4043 (2014)
  3. Kubota T, Arita M, Isobe Y, Iwamoto R, Goto T, Yoshioka T, Urabe D, Inoue M, Arai H.:
    “Eicosapentaenoic acid is converted via omega-3 epoxygenation to anti-inflammatory metabolite 12-hydroxy-17,18-epoxyeicosatetraenoic acid.”
    FASEB J 28, 586-593 (2014)
  4. *Morita M, Kuba K, Ichikawa A, Nakayama M, Katahira J, Iwamoto R, Watanabe T, Sakabe S, Daidoji T, Nakamura S, Kadowaki A, Ohto T, Nakanishi H, Taguchi R, Nakaya T, Murakami M, Yoneda Y, Arai H, Kawaoka Y, Penninger JM, Arita M, Imai Y.:
    “The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza.”
    Cell 153, 112-125 (2013)
  5. *Miyata J, Fukunaga K, Iwamoto R, Isobe Y, Niimi K, Takamiya R, Takihara T, Tomomatsu K, Suzuki Y, Oguma T, Sayama K, Arai H, Betsuyaku T, Arita M, Asano K.:
    “Dysregulated synthesis of protectin D1 in eosinophils from patients with severe asthma.”
    J Allergy Clin Immunol 131, 353-360 (2013)
  6. *Isobe Y, Arita M, Matsueda S, Iwamoto R, Fujihara T, Nakanishi H, Taguchi R, Masuda K, Sasaki K, Urabe D, Inoue M, Arai H.:
    “Identification and structure determination of novel anti-inflammatory mediator resolvin E3: 17,18-dihydroxyeicosapentaenoic acid.”
    J Biol Chem 287, 10525-10534 (2012)
  7. *Arita M.
    “Mediator lipidomics in acute inflammation and resolution. (review)”
    J Biochem 152, 313-319 (2012)
  8. *Yamada T, Tani Y, Nakanishi H, Taguchi R, Arita M, Arai H.:
    “Eosinophils promote resolution of acute peritonitis by producing pro-resolving mediators in mice.”
    FASEB J 25 561-568 (2011)
  9. *Schwab JM, Chiang N, Arita M, Serhan CN.:
    “Resolvin E1 and protectin D1 activate inflammation-resolution programmes.”
    Nature 447, 869-874 (2007)
  10. *Arita M, Bianchini F, Aliberti J, Sher A, Chiang N, Hong S, Yang R, Petasis NA, Serhan CN.:
    “Stereochemical assignment, anti-inflammatory properties, and receptor for the omega-3 lipid mediator resolvin E1.”
    J Exp Med 201, 713-722 (2005)

Lab Members

Principal Investigator

Makoto Arita
Team Leader

Core Members

Ken-ichi Moto
Senior Research Scientist
Kazutaka Ikeda
Senior Scientist
Tomoaki Ishihara
Research Scientist
Yosuke Isobe
Special Postdoctoral Researcher
Tatsuro Naganuma
Special Postdoctoral Researcher
Ryohei Aoyagi
Junior Research Associate
Mamoru Ogawa
Junior Research Associate
Mie Honda
Technical Staff I
Yuuya Senoo
Technical Staff I
Miwa Yoshimoto
Technical Staff I

Contact information

North Research Building,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Tel: +81-(0)45-503-7055
Fax: +81-(0)45-503-7054

Email: makoto.arita [at] riken.jp

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