Centers & Labs

RIKEN Center for Integrative Medical Sciences

Laboratory for Tissue Dynamics

Team Leader: Takaharu Okada (Ph.D.)
Takaharu  Okada(Ph.D.)

The goal of the laboratory is to understand the mechanisms regulating cell migration and interactions in the tissues that shape adaptive immune responses. For this purpose, we use real time imaging, in particular two-photon microscopy, to analyze cellular migration and interactions in the tissues. By applying the imaging strategy to relevant mouse models, we aim to reveal immune cell dynamics that are critical for generation of immunological memory and tolerance, two important features of adaptive immunity.

Main Research Field

Biological Sciences

Related Research Fields

Engineering / Biology / Medicine, dentistry, and pharmacy

Research Subjects

  • Follicular helper T cell dynamics and generation of humoral immune memory
  • Cellular interactions regulating differentiation of cytotoxic T Lymphocytes
  • Cellular interactions important for peripheral tolerance

Selected Publications

  1. Okada T., Moriyama S., and Kitano M.:
    "Differentiation of germinal center B cells and follicular helper T cells as viewed by tracking Bcl6 expression dynamics."
    Immunol Rev. 247: 120-132 (2012)
  2. Hirata E., Yukinaga H., Kamioka Y., Arakawa Y., Miyamoto S., Okada T., Sahai E., and Matsuda M.:
    "In vivo fluorescence resonance energy transfer imaging reveals differential activation of Rho-family GTPases in glioblastoma cell invasion."
    J Cell Sci. 125: 858-868 (2012)
  3. Kitano M., and Okada T.:
    "Four-dimensional tracking of lymphocyte migration and interactions in lymph nodes by two-photon microscopy."
    Methods Enzymol. 506: 437-454 (2012)
  4. Baumjohann D., Okada T., and Ansel K.M.:
    "Cutting Edge: Distinct waves of BCL6 expression during T follicular helper cell development."
    J Immunol. 187: 2089-2092 (2011)
  5. Kitano M., Moriyama S., Ando Y., Hikida M., Mori Y., Kurosaki T., and Okada T.:
    "Bcl6 protein expression shapes pre-germinal center B cell dynamics and follicular helper T cell heterogeneity."
    Immunity. 34: 961-72 (2011)
  6. Tanizaki H., Egawa G., Inaba K., Honda T., Nakajima S., Moniaga C.S., Otsuka A., Ishizaki T., Tomura M., Watanabe T., Miyachi Y., Narumiya S., Okada T., and Kabashima K.:
    "Rho-mDia1 pathway is required for adhesion, migration, and T-cell stimulation in dendritic cells."
    Blood 116: 5875-5884 (2010)
  7. Okada T.:
    "Two-photon microscopy analysis of leukocyte trafficking and motility."
    Semin. Immunopathol. 32: 215-225 (2010)
  8. Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, and Kinashi T.:
    "Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes."
    EMBO J. 28: 1319-1331 (2009)
  9. Allen, C.D., Okada, T., Tang, H.L., and Cyster, J.G.:
    "Imaging of germinal center selection events during affinity maturation."
    Science, 315, 528-531, (2007)
  10. Okada, T., Miller, M.J., Parker, I., Krummel, M.L., Neighbors, M., Hartley, S.B., O’Garra, A., Cahalan, M.D., and Cyster, J.G.:
    "Antigen-engaged B cells undergo chemotaxis toward the T zone and form motile conjugates with helper T cells."
    PLoS Biology 3: 1047-1061(e150) (2005)

Lab Members

Principal Investigator

Takaharu Okada
Team Leader

Core Members

Hidehiro Fukuyama
Deputy Team Leader
Harumichi Ishigame
Research Scientist
Noriko Takahashi
Technical Staff I
Akiko Takumi
Technical Staff I

Contact information

1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan

Email: takaharu.okada [at] riken.jp

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