1. Home
  2. Research
  3. Centers & Labs
  4. RIKEN Center for Biosystems Dynamics Research

RIKEN Center for Biosystems Dynamics Research Human Biomimetic System RIKEN Hakubi Research Team

RIKEN Hakubi Team Leader: Masaya Hagiwara (Ph.D.)

Research Summary

Masaya  Hagiwara

The overall objective is the development of in-vitro experimental platform to reconstitute the biological system outside our bodies by controlling cellular micro-environments. Especially lung airway is the one of my major targets. The scientific question is how the airway cells can organize such a sophisticated structure by themselves. The repetitive feedbacks between in-vitro experiments and mathematical model give us a tremendous amount of useful information, and I am challenging to unveil the system underlying self-organization of tissue formation by directing complex tissue structure. Development of Organ or Body-on-a-Chip system is another research target for the animal testing alternative.

Main Research Fields

  • Complex Systems

Related Research Fields

  • Informatics
  • Interdisciplinary Science & Engineering
  • Mathematical & Physical Sciences
  • Chemistry
  • Engineering
  • Biological Sciences
  • Biology
  • Medicine, Dentistry & Pharmacy
  • Biomedical engineering/Biomaterial science and engineering
  • Nano/Microsystems
  • Intelligent mechanics/Mechanical systems


  • In vitro tissue formation
  • Tissue imaging
  • Organ on a Chip
  • Culture Control

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1. Takano, A., Koh, I., and Hagiwara, M.:
    "3D Culture Platform for Enabling Large-Scale Imaging and Control of Cell Distribution into Complex Shapes by Combining 3D Printing with a Cube Device"
    Micromachines, 13, 156, (2022)
  • 2. Hagiwara, M., Maruyama, H., Akiyama, M., Koh, I., Arai, F.:
    "Weakening of resistance force by cell–ECM interactions regulate cell migration directionality and pattern formation"
    Communications Biology volume 4, 808 (2021)
  • 3. Hagiwara, M., Koh, I.:
    "Engineering approaches to control and design the in vitro environment towards the reconstruction of organs"
    Development, Growth & Differentiation, 62, pp158-166 (2020)
  • 4. *Hagiwara, M., Nobata, R., Kawahara, T.;
    "Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations"
    Journal of Visualized Experiments, 145, e5924, (2019)
  • 5. *Hagiwara, M., Nakase, I.:
    "Epidermal growth factor induced macropinocytosis directs branch formation of lung epithelial cells"
    Biochemical and Biophysical Research Communications, 507, 297-303, (2018).
  • 6. *Hagiwara, M., Nobata, R., Kawahara, T.:
    "High repeatability from 3D experimental platform for quantitative analysis of cellular branch pattern formations"
    Integrative Biology, 10, 306-312, (2018).
  • 7. *Hagiwara, M., Nobata, R., Kawahara, T.:
    "Large scale imaging by fine spatial alignment of multi-scanning data with gel cube device"
    Applied Sciences, 8, doi:10.3390/app8020235, (2018).
  • 8. *Hagiwara, M., Maruta, M., Marumoto, M.:
    "In Vitro Experimental Model for the Long-Term Analysis of Cellular Dynamics During Bronchial Tree Development from Lung Epithelial Cells"
    Tissue Engineering Part C: Methods, 23, 323-332, (2017).
  • 9. *Hagiwara, M:
    "An in vitro–in silico interface platform for spatiotemporal analysis of pattern formation in collective epithelial cells"
    Integrative Biology, 8, 861-868, (2016).
  • 10. *Hagiwara, M., Nobata, R., Kawahara, T.:
    "Tissue in Cube: In Vitro 3D Culturing Platform with Hybrid Gel Cubes for Multidirectional Observations"
    Advanced Healthcare Materials, 5, 1566-1571, (2016).

Recent Research Results

Lab Members

Principal investigator

Masaya Hagiwara
RIKEN Hakubi Team Leader

Core members

Raja Kumar Vadivelu
Special Postdoctoral Researcher
Isabel Siew Yin Koh
Postdoctoral Researcher
Yen Xuan Ngo
Postdoctoral Researcher
Satoko Kinoshita

Contact Information

Bioscience Bldg.
2-1 Hirosawa,
Wako, Saitama,
351-0198, Japan
Email: masaya.hagiwara [at] riken.jp