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RIKEN BioResource Research Center (BRC®) Drug Discovery Genetically Modified Animal Platform Unit

Unit Leader: Takanori Amano (Ph.D.)

Research Summary

Takanori Amano

With the accumulation of patient genomic information related to diseases, elucidating the relationship between genomes and diseases using animal models plays a crucial role in preclinical research and drug discovery. This unit utilizes genome editing technology to generate genetically modified mice and conducts detailed phenotyping in vivo to validate disease relevance and assess the effectiveness of drug targets. Furthermore, we refine genome editing and phenotypic analysis techniques to develop humanized disease model mice that contribute to the advancement of personalized medicine, including antibody and nucleic acid therapies.

Main Research Fields

  • Biology

Related Research Fields

  • Medicine, Dentistry & Pharmacy
  • Genetics-related
  • Medical biochemistry-related
  • Genome biology-related

Keywords

  • Disease Model
  • Genome
  • Genetic Disease
  • Drug Discovery
  • Genome Editing

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1. Saito A, Tahara R, Hirose M, Kadota M, Hasegawa A, Kondo S, Kato H, Amano T, Yoshiki A, Ogura A and Kiyosawa H.:
    "Inter-subspecies mouse F1 hybrid embryonic stem cell lines newly established for studies of allelic imbalance in gene expression"
    Experimental Animals 24-0002. (2024).
  • 2. Kawashima H, Nishimata S, Shinji S, Morishima Y, Tsutsumi N, Kashiwagi K, Amano T, Tamura M, Ayabe S and Nakashima K.
    "Three Siblings with Aarskog Scott Syndrome Together with Mental Retardation and Giant Megacolon"
    Glob. J. Pedia. 2, 1, IFN1000278. (2024).
  • 3. Tada R+, Higashidate T+, Amano T, Ishikawa S, Yokoyama C, Kobari S, Nara S, Ishida K, Kawaguchi A, Ochi H, Ogino H, Yakushiji-Kaminatsui N, Sakamoto J, Kamei Y, Tamura K and Yokoyama A.
    "The shh limb enhancer is activated in patterned limb regeneration but not in hypomorphic limb regeneration in Xenopus laevis"
    Developmental Biology 500, 22-30. (2023).
  • 4. Tanaka S, Orita H, Kataoka T, Miyazaki M, Saeki H, Wada R, Brock MV, Fukunaga T, Amano T, and Shiroishi T
    "Gasdermin D Represses Inflammation-Induced Colon Cancer Development by Regulating Apoptosis"
    Carcinogenesis 44, 341–349. (2023).
  • 5. Amano T
    "Gene regulatory landscape of the sonic hedgehog locus in embryonic development"
    Dev. Growth Differ. 62, 334-342. (2020).
  • 6. Sagai T, Amano T, Maeno A, Ajima R, and Shiroishi T.
    "SHH signaling mediated by a prechordal and brain enhancer controls forebrain organization"
    Proc. Natl. Acad. Sci. USA 116, 23636-23642. (2019).
  • 7. *Seo H+, Amano T+, Seki R, Sagai T, Kim J, Cho SW and Shiroishi T.
    "Upstream Enhancer Elements of Shh Regulate Oral and Dental Patterning."
    J. Dent. Res. 97, 1055-1063. (2018).
  • 8. *Mouri K, Sagai T, Maeno A, Amano T, Toyoda A and Shiroishi T.
    "Enhancer Adoption Caused by Genomic Insertion Elicits Interdigital Shh Expression and Syndactyly in Mouse."
    Proc. Natl. Acad. Sci. U. S. A. 115, 1021-1026. (2018).
  • 9. *Sagai T+, Amano T+, Maeno A, Kiyonari H, Seo H, Cho SW and Shiroishi T.
    "SHH Signaling Directed by Two Oral Epithelium-Specific Enhancers Controls Tooth and Oral Development."
    Sci. Rep. 7, 13004. (2017).
  • 10. *Amano T, Sagai T, Seki R and Shiroishi T.
    "Two Types of Etiological Mutation in the Limb-Specific Enhancer of Shh."
    G3 7, 2991-2998. (2017)

Lab Members

Principal investigator

Takanori Amano
Unit Leader

Contact Information

3-1-1 Koyadai
Tsukuba, Ibaraki
305-0074 Japan
Research Building (2nd Phase)
Tel: +81-(0)29-846-0048
Email: takanori.amano@riken.jp

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