RIKEN BioResource Research Center (BRC®) iPS Cell Advanced Characterization and Development Team
Team Leader: Toshihiko Shiroishi (Ph.D.)
Research Summary
- Advanced characterization (e.g., pluripotency and genome) of disease-specific iPS cells deposited in RIKEN cell bank
- Development of modified (e.g., mutation-corrected, mutation-introduced, and reporter-introduced) iPS cells using genome-editing technologies
- Development of differentiation system and disease models using disease-specific iPS cells deposited in RIKEN cell bank
Main Research Fields
- Medicine, Dentistry & Pharmacy
Related Research Fields
- Biology
Keywords
- Disease-specific iPS Cells
- Genome Editing
- Disease Models
- Cell Bank
- Regenerative Medicine
Selected Publications
- 1.
Matsuo-Takasaki, M., Kambayashi, S., Hemmi, Y., Wakabayashi, T., Shimizu, T., An, Y., Ito, H., Takauchi, K., Ibuki, M., Kawashima, T., Masayasu, R., Suzuki, M., Kawai, Y., Umekage, M., Kato, TM., Noguchi, M., Nakade, K., Nakamura, Y., Nakaishi, T., Nishishita, N., Tsukahara, M., and Hayashi, Y.:
"Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation"
eLife: doi.org/10.7554/eLife.89724.3 (2024). - 2.
Takagi, D., Tsukamoto, S., Nakade, K., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Yumoto, N., Kawada, J., Hayata, T., and Hayashi, Y.:
"Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization."
Stem Cell Res 79:103493 (2024). - 3.
Mori, M., Yoshii, S., Noguchi, M., Takagi, D., Shimizu, T., Ito, H., Matsuo-Takasaki, M., Nakamura, Y., Takahashi, S., Hamada, H., Ohnuma, K., Shiohama, T., and Hayashi, Y.:
"Generation of human induced pluripotent stem cell lines derived from four Rett syndrome patients with MECP2 mutations."
Stem Cell Res 77:103432 (2024). - 4.
Song, D., Takahashi, G., Zheng, YW., Matsuo-Takasaki, M., Li, J., Takami, M., An, Y., Hemmi, Y., Miharada, N., Fujioka, T., Noguchi, M., Nakajima, T., Saito, MK., Nakamura, Y., Oda, T., Miyaoka, Y., and Hayashi, Y.:
"Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes."
Hum Mol Genet 31(21):3652-3671 (2022). - 5.
Shimizu, T., Matsuo-Takasaki, M., Luijkx, D., Takami, M., Arai, Y., Noguchi, M., Nakamura, Y., Hayata, T., Saito, MK., and Hayashi, Y.:
"Generation of human induced pluripotent stem cell lines derived from four DiGeorge syndrome patients with 22q11.2 deletion."
Stem Cell Res 61:102744 (2022). - 6.
Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, TA., Yumoto, F., Terada, T., Hisatake, K., and Hayashi, Y.:
"Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency."
iScience 25(1):103525 (2021). - 7.
Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., and Hayashi, Y.:
Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing."
"Stem Cell Res 53:102363 (2021). - 8.
Kuramochi, Y., Awaya, T., Matsuo-Takasaki, M., Takami, M., An, Y., Li, J., Hemmi, Y., Wakabayashi, T., Arai, Y., Inoue, J., Noguchi, M., Nakamura, Y., Asaka, I., Akimoto, K., Saito, MK., and Hayashi, Y.:
"Generation of two human induced pluripotent stem cell lines derived from two X-linked adrenoleukodystrophy patients with ABCD1 mutations."
Stem Cell Res 53:102337 (2021). - 9.
Arai, Y., Takami, M., An, Y., Matsuo-Takasaki, M., Hemmi, Y., Wakabayashi, T., Inoue, J., Noguchi, M., Nakamura, Y., Sugimoto, K., Takemura, T., Okita, K., Osafune, K., Takasato, M., Hayata, T., and Hayashi, Y.:
"Generation of two human induced pluripotent stem cell lines derived from two juvenile nephronophthisis patients with NPHP1 deletion."
Stem Cell Res 45:101815 (2020).
Lab Members
Principal investigator
- Toshihiko Shiroishi
- Team Leader
Core members
- Mami Takasaki
- Research & Development Scientist
- Evgeniia Borisova
- Junior Research Associate
- Tamami Wakabayashi
- Technical Staff II
- Yasuko Henmi
- Technical Staff II