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RIKEN BioResource Research Center (BRC®) iPS Cell Advanced Characterization and Development Team

Team Leader: Toshihiko Shiroishi (Ph.D.)

Research Summary

Toshihiko Shiroishi
  • Advanced characterization (e.g., pluripotency and genome) of disease-specific iPS cells deposited in RIKEN cell bank
  • Development of modified (e.g., mutation-corrected, mutation-introduced, and reporter-introduced) iPS cells using genome-editing technologies
  • Development of differentiation system and disease models using disease-specific iPS cells deposited in RIKEN cell bank

Main Research Fields

  • Medicine, Dentistry & Pharmacy

Related Research Fields

  • Biology

Keywords

  • Disease-specific iPS Cells
  • Genome Editing
  • Disease Models
  • Cell Bank
  • Regenerative Medicine

Selected Publications

  • 1. Matsuo-Takasaki, M., Kambayashi, S., Hemmi, Y., Wakabayashi, T., Shimizu, T., An, Y., Ito, H., Takauchi, K., Ibuki, M., Kawashima, T., Masayasu, R., Suzuki, M., Kawai, Y., Umekage, M., Kato, TM., Noguchi, M., Nakade, K., Nakamura, Y., Nakaishi, T., Nishishita, N., Tsukahara, M., and Hayashi, Y.:
    "Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation"
    eLife: doi.org/10.7554/eLife.89724.3 (2024).
  • 2. Takagi, D., Tsukamoto, S., Nakade, K., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Yumoto, N., Kawada, J., Hayata, T., and Hayashi, Y.:
    "Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization."
    Stem Cell Res 79:103493 (2024).
  • 3. Mori, M., Yoshii, S., Noguchi, M., Takagi, D., Shimizu, T., Ito, H., Matsuo-Takasaki, M., Nakamura, Y., Takahashi, S., Hamada, H., Ohnuma, K., Shiohama, T., and Hayashi, Y.:
    "Generation of human induced pluripotent stem cell lines derived from four Rett syndrome patients with MECP2 mutations."
    Stem Cell Res 77:103432 (2024).
  • 4. Song, D., Takahashi, G., Zheng, YW., Matsuo-Takasaki, M., Li, J., Takami, M., An, Y., Hemmi, Y., Miharada, N., Fujioka, T., Noguchi, M., Nakajima, T., Saito, MK., Nakamura, Y., Oda, T., Miyaoka, Y., and Hayashi, Y.:
    "Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes."
    Hum Mol Genet 31(21):3652-3671 (2022).
  • 5. Shimizu, T., Matsuo-Takasaki, M., Luijkx, D., Takami, M., Arai, Y., Noguchi, M., Nakamura, Y., Hayata, T., Saito, MK., and Hayashi, Y.:
    "Generation of human induced pluripotent stem cell lines derived from four DiGeorge syndrome patients with 22q11.2 deletion."
    Stem Cell Res 61:102744 (2022).
  • 6. Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, TA., Yumoto, F., Terada, T., Hisatake, K., and Hayashi, Y.:
    "Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency."
    iScience 25(1):103525 (2021).
  • 7. Tsukamoto, S., Nakade, K., Wakabayashi, T., Nakashima, K., Takami, M., Hemmi, Y., Kuramochi, Y., Shimizu, T., Arai, Y., Matsuo-Takasaki, M., Noguchi, M., Nakamura, Y., Miwa, Y., and Hayashi, Y.:
    Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing."
    "Stem Cell Res 53:102363 (2021).
  • 8. Kuramochi, Y., Awaya, T., Matsuo-Takasaki, M., Takami, M., An, Y., Li, J., Hemmi, Y., Wakabayashi, T., Arai, Y., Inoue, J., Noguchi, M., Nakamura, Y., Asaka, I., Akimoto, K., Saito, MK., and Hayashi, Y.:
    "Generation of two human induced pluripotent stem cell lines derived from two X-linked adrenoleukodystrophy patients with ABCD1 mutations."
    Stem Cell Res 53:102337 (2021).
  • 9. Arai, Y., Takami, M., An, Y., Matsuo-Takasaki, M., Hemmi, Y., Wakabayashi, T., Inoue, J., Noguchi, M., Nakamura, Y., Sugimoto, K., Takemura, T., Okita, K., Osafune, K., Takasato, M., Hayata, T., and Hayashi, Y.:
    "Generation of two human induced pluripotent stem cell lines derived from two juvenile nephronophthisis patients with NPHP1 deletion."
    Stem Cell Res 45:101815 (2020).

Lab Members

Principal investigator

Toshihiko Shiroishi
Team Leader

Core members

Mami Takasaki
Research & Development Scientist
Evgeniia Borisova
Junior Research Associate
Tamami Wakabayashi
Technical Staff II
Yasuko Henmi
Technical Staff II

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