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RIKEN BioResource Research Center iPS Cell Advanced Characterization and Development Team

Team Leader: Yohei Hayashi (Ph.D.)

Research Summary

Yohei  Hayashi(Ph.D.)
  • Advanced characterization (e.g., pluripotency and genome) of disease-specific iPS cells deposited in RIKEN cell bank
  • Development of modified (e.g., mutation-corrected, mutation-introduced, and reporter-introduced) iPS cells using genome-editing technologies
  • Development of differentiation system and disease models using disease-specific iPS cells deposited in RIKEN cell bank

Main Research Fields

  • Medicine, Dentistry & Pharmacy

Related Research Fields

  • Biology


  • Disease-specific iPS Cells
  • Genome Editing
  • Disease Models
  • Cell Bank
  • Regenerative Medicine

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1.*Hayashi Y+, Hsiao EC, Sami S, Lancero M, Schlieve CR, Nguyen T, Yano K, Nagahashi A, Ikeya M, Matsumoto Y, Nishimura K, Fukuda A, Hisatake K, Tomoda K, Asaka I, Toguchida J, Conklin BR, and Yamanaka S.:
    (+ :Corresponding Author)
    “BMP-SMAD-ID Promotes Reprogramming to Pluripotency by Inhibiting p16/INK4A-Dependent Senescence.”
    Proc. Natl. Acad. Sci. USA 113(46): 13057-62 (2016).
  • 2.*Hayashi Y+, Furue MK.:
    (+ :Corresponding Author)
    “Biological Effects of Culture Substrates on Human Pluripotent Stem Cells”
    Stem Cells International 5380560 (2016).
  • 3.*Hayashi Y*, Caboni L*, Das D, Yumoto F, Clayton T, Deller MC, Nguyen P, Farr CL, Chiu HJ, Miller M, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Tomoda K, Conklin BR, Wilson IA, Yamanaka S, Fletterick, RJ.:
    (* :Equally Contributed Authors)
    “Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells.”
    Proc Natl Acad Sci U S A 112(15): 4666-71 (2015).
  • 4.*Bershteyn M*, Hayashi Y*, Desachy G, Hsiao EC, Sami S, Tsang KMJ, Weiss LA, Kriegstein AR, Yamanaka S, Wynshaw-Boris A.:
    (* :Equally Contributed Authors)
    “Cell-Autonomous Correction of Ring Chromosomes in Human Induced Pluripotent Stem Cells.”
    Nature 507(7490): 99-103 (2014).
  • 5.*Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, Narita M, Srivastava D, Yamanaka S.:
    “The let-7 Target LIN-41 Promotes Induced Pluripotent Stem Cell Reprogramming.”
    Cell Stem Cell 14(1): 40-52 (2014).
  • 6.*Hayashi Y+, Saitou M, Yamanaka S.
    (+ :Corresponding Author)
    “Germline Development from Human Pluripotent Stem Cells toward Disease Modeling of Infertility.”
    Fertil Steril 97(6): 1250-9 (2012).
  • 7.*Ieda M, Fu JD, Delgado-Olguin P, Vedantham V, Hayashi Y, Bruneau BG, Srivastava, D.:
    “Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors.”
    Cell 142(3): 375-86 (2010).
  • 8.*Hayashi Y*, Chan T*, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, Ohnuma K, Nakauchi H, Asashima M.:
    (* :Equally Contributed Authors)
    “Reduction of N-Glycolylneuraminic Acid in Human Induced Pluripotent Stem Cells Generated or Cultured under Feeder- and Serum-Free Defined Conditions.”
    PLoS One 5(11): e14099 (2010).
  • 9.*Hayashi Y, Furue MK, Tanaka S, Hirose M, Wakisaka N, Danno H, Ohnuma K, Oeda S, Aihara Y, Shiota K, Ogura A, Ishiura S, Asashima M.:
    “BMP4 induction of trophoblast from mouse embryonic stem cells in defined culture conditions on laminin.”
    In Vitro Cell Dev Biol Anim 46(5): 416-30 (2009).
  • 10.*Hayashi Y, Furue M, Okamoto T, Ohnuma K, Myoishi Y, Fukuhara Y, Abe T, Sato JD, Hata RI, Asashima M.:
    “Integrin regulates mouse embryonic stem cell self-renewal.”
    Stem Cells 25(12): 3005-15 (2007).

Related Links

Lab Members

Principal investigator

Yohei Hayashi
Team Leader

Core members

Mami Takasaki
Research & Development Scientist
Evgeniia Borisova
Junior Research Associate
Tamami Wakabayashi
Technical Staff II
Yasuko Henmi
Technical Staff II

Contact Information

BioResource Building for Cell Research,
3-1-1 Koyadai,
Tsukuba, Ibaraki,
305-0074, Japan

Email: yohei.hayashi [at] riken.jp