Research Summary

Selected Publications

• 1. Abe, M., Yanagawa, M., Hiroshima, M., Kobayashi, T., and Sako, Y.:
  "Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopy"
  eLife :e101652 (2024).
• 2. Okamoto, K. and Sako, Y.:
  "Two closed conformations of CRAF require the 14-3-3 binding motifs and cysteine-rich domain to be intact in live cells"
  J. Mol. Biol. 435, 167989 (2023).
• 3. Maeda, R., Tamakgaki-Asahina, H., Sato, T., Yanagawa, M., and Sako, Y.:
  "Threonine phosphorylation regulates the molecular assembly and signaling of EGFR in cooperation with membrane lipids"
  J. Cell Sci. 135, jcs260355 (1-12) (2022).
• 4. Yoshizawa, R., Umeki, N., Yamamoto, A., Okada, M., Murata, M., and Sako, Y.:
  "p52Shc regulates the sustainability of ERK activation in a RAF-independent manner"
  Mol. Biol. Cell. 32, 1838-1848 (2021).
• 5. Okamoto, K., Hibino, K., and Sako, Y.:
  "In-cell single-molecule FRET measurements reveal three conformational state changes in RAF protein"
  Biochim. Biophys. Acta. Gen. Subj. 1864(2), 129358 (2020).
• 6. Yanagawa, M., Hiroshima, M., Togashi, Y., Abe, M., Yamashita, T., Shichida, Y., Murata, M., Ueda, M., and Sako, Y.:
  "Single-molecule diffusion-based estimation of ligand effects on G protein-coupled receptors"
  Sci. Signal. 11(548), eaao1917 (2018).
• 7. Hiroshima, M., Pack, C.-G., Kaizu, K., Takahashi, K., Ueda, M., and Sako, Y.:
  "Transient acceleration of epidermal growth factor receptor dynamics produces higher-order signaling clusters"
  J. Mol. Biol. 430(9), 1386-1401 (2018).
• 8. Maeda, R., Sato, T., Okamoto, K., Yanagawa, M., and Sako, Y.:
  "Lipid-protein interplay in dimerization of juxtamembrane domains of epidermal growth factor receptor"
  Biophys. J. 114(4), 893-903 (2018).
• 9. Nakamura, Y., Umeki, N., Abe, M., and Sako, Y.:
  "Mutation-specific mechanisms of hyperactivation of Noonan syndrome SOS molecules detected with single-molecule imaging in living cells"
  Sci. Rep. 7(1), 14153 (2017).
• 10. Arata, Y., Hiroshima, M., Pack, C.-G., Ramanujam, R., Motegi, F., Nakazato, K., Wiseman, P. W., Sawa, H., Kobayashi, T. J., Brandão, H. B., Shibata, T., and Sako, Y.:
  "Cortical polarity of the RING protein PAR-2 is maintained by exchange rate kinetics at the cortical-cytoplasm boundary"
  Cell Rep. 16(8), 2156-2168 (2016).

Recent Research Results

• 

  Apr. 17, 2025
  Lipid ‘nanodomains’ hold the key to cell signaling mystery

• 

  Nov. 30, 2018
  Movement patterns of receptors can indicate their activation status

• 

  Jul. 11, 2016
  Learning from the mussel, scientists create a biologically active titanium surface

Annual research report

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