1. Home
  2. Research
  3. Centers & Labs
  4. RIKEN Center for Sustainable Resource Science
  5. Technology Platform Division

RIKEN Center for Sustainable Resource Science Mass Spectrometry and Microscopy Unit

Unit Leader: Masami Hirai (Ph.D.)

Research Summary

Masami Hirai (Ph.D.)

Mass spectrometric and microscopic analyses are fundamental analytical technology in plant science and sustainable resource science. Our unit develops and executes the analyses based on mass spectrometry for the study of plant metabolome and hormonome and on microscopy for the ultrastructural observation of the plant cells.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Chemistry
  • Biology
  • Agricultural Sciences
  • Medicine, Dentistry & Pharmacy

Keywords

  • metabolomics
  • phytohormone
  • mass spectrometry
  • microscopy
  • phenomics

Selected Publications

  • 1. Goto, Y., Takeda-Kamiya, N., Yamaguchi, K., Yamazaki, M. and Toyooka, K.:
    "Effective alignment method using a diamond notch knife for correlative array tomography”
    Microscopy 73, 446-450. (2024)
  • 2. Soyano, T., Akamatsu, A., Takeda, N., Watahiki, M. K., Goh, T., Okuma, N., Suganuma, N., Kojima, M., Takebayashi, Y., Sakakibara, H., Nakajima, K., and Kawaguchi, M.:
    "Periodic cytokinin responses in Lotus japonicus rhizobium infection and nodule development”
    Science 385,288-294. (2024)
  • 3. Shoji, T., Sugawara, S., Mori, T., Kobayashi, M., Kusano, M. and Saito, K.:
    "Induced production of specialized steroids by transcriptional reprogramming in Petunia hybrida”
    PNAS Nexus 2, (2023)
  • 4. Toyooka, K., Goto, Y., Hashimoto, K., Wakazaki, M., Sato, M. and Hirai, M.Y.:
    "Endoplasmic Reticulum Bodies in the Lateral Root Cap are Involved in the Direct Transport of Beta-Glucosidase to Vacuoles”
    Plant Cell Physiology 64, 461-473. (2023)
  • 5. Kojima, M., Makita, N., Miyata, K., Yoshino, M., Iwase, A., Ohashi, M., Surjana, A., Kudo, T., Takeda-Kamiya, N., Toyooka, K., Miyao, A., Hirochika, H., Ando, T., Shomura, A., Yano, M., Yamamoto, T., Hobo, T., and Sakakibara, H.:
    "A cell wall–localized cytokinin/purine riboside nucleosidase is involved in apoplastic cytokinin metabolism in Oryza sativa”
    PNAS 120, (2023)
  • 6. Zhang, J., Wan, W., Tanaka, N., Fujita, M., Takahashi, K. and Harada, K.:
    "Integrating a Pipette into a Robot Manipulator with Uncalibrated Vision and TCP for Liquid Handling”
    IEEE TRANSACTIONS ON AUTOMATION SCIENCE AND ENGINEERING, 21, 5503–5522. (2023).
  • 7. Nagatoshi, Y., Ikazaki, K., Kobayashi, Y., Mizuno, N., Sugita, R., Takebayashi, Y., Kojima, M., Sakakibara, H., Kobayashi, N. I., Tanoi, K., Fujii, K., Baba, J., Ogiso-Tanaka, E., Ishimoto, M., Yasui, Y., Oya, T., and Fujita, Y.:
    "Phosphate starvation response precedes abscisic acid response under progressive mild drought in plants”
    Nat Commun 14, 5047. (2022)
  • 8. Kusano, M., Fukushima, A., Tabuchi-Kobayashi, M., Funayama, K., Kojima, S., Maruyama, K., Yamamoto,Y.Y., Nishizawa, T., Kobayashi, M., Wakazaki, M., Sato, M., Toyooka, K., Osanai-Kondo, K., Utsumi, Y., Seki, M., Fukai, C., Saito, K. and Yamaya, T.:
    "Cytosolic GLUTAMINE SYNTHETASE 1;1 Modulates Metabolism and Chloroplast Development in Roots”
    Plant Physiol. 182, 1894–1909. (2020)
  • 9. Tsugawa, H., Ikeda, K., Takahashi, M., Satoh, A., Mori, Y., Uchino, H., Okahashi, N., Yamada, Y., Tada, I., Bonini, P., Higashi, Y., Okazaki, Y., Zhou, Z., Zhu, Z.J., Koelmel, J., Cajka, T., Fiehn, O., Saito, K., Arita, M. and Arita, M.:
    "A lipidome atlas in MS-DIAL 4”
    Nat Biotechnol.38, 1159-1163. (2020)
  • 10. Fujita, M., Tanabata, T., Urano, K., Kikuchi, S., and Shinozaki, K.:
    "RIPPS: A Plant Phenotyping System for Quantitative Evaluation of Growth Under Controlled Environmental Stress Conditions”
    Plant Cell Physiol. 59, 2030-2038. (2018)

Related Links

Lab Members

Principal investigator

Masami Hirai
Unit Leader

Core members

Kiminori Toyooka
Senior Technical Scientist
Miki Fujita
Senior Technical Scientist
Mayuko Sato
Technical Scientist
Mikiko Kojima
Expert Technician
Ryosuke Sasaki
Expert Technician
Tetsuya Mori
Expert Technician
Makoto Kobayashi
Technical Staff I
Muneo Sato
Technical Staff I
Yumiko Takebayashi
Technical Staff I
Yumi Goto
Technical Staff II
Kouji Takano
Technical Staff II
Noriko Takeda
Technical Staff II
Mayumi Wakazaki
Technical Staff II
Akira Oikawa
Senior Visiting Scientist
Miyako Kusano
Senior Visiting Scientist
Hitoshi Sakakibara
Senior Visiting Scientist
Tetsuya Sakurai
Senior Visiting Scientist
Mitsunori Seo
Senior Visiting Scientist

Contact Information

1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan

Email: masami.hirai@riken.jp

Top