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RIKEN Center for Sustainable Resource Science Mass Spectrometry and Microscopy Unit

Unit Leader: Masami Hirai (Ph.D.)

Research Summary

Masami Hirai (Ph.D.)

Mass spectrometric and microscopic analyses are fundamental analytical technology in plant science and sustainable resource science. Our unit develops and executes the analyses based on mass spectrometry for the study of plant metabolome and hormonome and on microscopy for the ultrastructural observation of the plant cells.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Chemistry
  • Biology
  • Agricultural Sciences
  • Medicine, Dentistry & Pharmacy

Keywords

  • metabolomics
  • phytohormone
  • mass spectrometry
  • microscopy

Selected Publications

  • 1.Nakabayashi, R., Hashimoto, K., Toyooka, K. and Saito, K.:
    "Top-down Metabolomic Approaches for Nitrogen-Containing Metabolites"
    Analytical Chemistry, 89, 2698-2703 (2017)
  • 2.Sawada, Y., Tsukaya, H., Li, Y., Sato, M., Kawade, K. and Hirai, M.Y.:
    "A Novel Method for Single-grain-based Metabolic Profiling of Arabidopsis Seed"
    Metabolomics, 13, 75 (2017)
  • 3.Toyooka, K.:
    "Development of correlative light and electron microscopy to observe green fluorescent protein-labeled organelles embedded in resin using field-emission electron scanning microscope"
    Plant Morphology, 28, 15-21 (2016)
  • 4.Toyooka, K., Sato, M., Kutsuna, N., Higaki, T., Sawaki, F., Wakazaki, M., Goto, Y., Hasezawa, S., Nagata, N. and Matsuoka, K.:
    "Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log-to-stationary transition of growth phase in tobacco BY-2 cells"
    Plant Cell Physiology, 55, 1544-1555 (2014)
  • 5.Nakabayashi, R., Sawada, Y., Yamada, Y., Suzuki, M., Hirai, M.Y., Sakurai, T and Saito, K.:
    "Combination of Liquid Chromatography-Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry with 13C-labeling for Chemical Assignment of Sulfur-containing Metabolites in Onion Bulbs"
    Analytical Chemistry, 85, 1310–1315 (2013)
  • 6.Sawada, Y. and Hirai, M.Y.:
    "Integrated LC-MS/MS system for plant metabolomics"
    Computational and Structural Biotechnology Journal, 4, e20131011. (2013)
  • 7.Kojima, M., Kamada-Nobusada, T., Komatsu, H., Takei, K., Kuroha, T., Mizutani, M., Ashikari, M., Ueguchi-Tanaka, M., Matsuoka, M., Suzuki, K. and Sakakibara, H.:
    "Highly-sensitive and High-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: an application for hormone profiling in Oryza sativa"
    Plant Cell Physiology, 50, 1201-1214 (2009)

Related Links

Lab Members

Principal investigator

Masami Hirai
Unit Leader

Core members

Kiminori Toyooka
Senior Technical Scientist
Yasuhiro Higashi
Research Scientist
Ryo Nakabayashi
Research Scientist
Mayuko Sato
Technical Scientist
Mikiko Kojima
Expert Technician
Ryosuke Sasaki
Expert Technician
Tetsuya Mori
Expert Technician
Makoto Kobayashi
Technical Staff I
Muneo Sato
Technical Staff I
Yumiko Takebayashi
Technical Staff I
Yumi Goto
Technical Staff II
Kouji Takano
Technical Staff II
Noriko Takeda
Technical Staff II
Mayumi Wakazaki
Technical Staff II

Contact Information

1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan

Email: masami.hirai [at] riken.jp

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