RIKEN Center for Sustainable Resource Science Mass Spectrometry and Microscopy Unit
Unit Leader: Masami Hirai (Ph.D.)
Research Summary
Mass spectrometric and microscopic analyses are fundamental analytical technology in plant science and sustainable resource science. Our unit develops and executes the analyses based on mass spectrometry for the study of plant metabolome and hormonome and on microscopy for the ultrastructural observation of the plant cells.
Main Research Fields
- Biological Sciences
Related Research Fields
- Chemistry
- Biology
- Agricultural Sciences
- Medicine, Dentistry & Pharmacy
Keywords
- metabolomics
- phytohormone
- mass spectrometry
- microscopy
Selected Publications
- 1.Nakabayashi, R., Hashimoto, K., Toyooka, K. and Saito, K.:
"Top-down Metabolomic Approaches for Nitrogen-Containing Metabolites"
Analytical Chemistry, 89, 2698-2703 (2017) - 2.Sawada, Y., Tsukaya, H., Li, Y., Sato, M., Kawade, K. and Hirai, M.Y.:
"A Novel Method for Single-grain-based Metabolic Profiling of Arabidopsis Seed"
Metabolomics, 13, 75 (2017) - 3.Toyooka, K.:
"Development of correlative light and electron microscopy to observe green fluorescent protein-labeled organelles embedded in resin using field-emission electron scanning microscope"
Plant Morphology, 28, 15-21 (2016) - 4.Toyooka, K., Sato, M., Kutsuna, N., Higaki, T., Sawaki, F., Wakazaki, M., Goto, Y., Hasezawa, S., Nagata, N. and Matsuoka, K.:
"Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log-to-stationary transition of growth phase in tobacco BY-2 cells"
Plant Cell Physiology, 55, 1544-1555 (2014) - 5.Nakabayashi, R., Sawada, Y., Yamada, Y., Suzuki, M., Hirai, M.Y., Sakurai, T and Saito, K.:
"Combination of Liquid Chromatography-Fourier Transform-Ion Cyclotron Resonance-Mass Spectrometry with 13C-labeling for Chemical Assignment of Sulfur-containing Metabolites in Onion Bulbs"
Analytical Chemistry, 85, 1310–1315 (2013) - 6.Sawada, Y. and Hirai, M.Y.:
"Integrated LC-MS/MS system for plant metabolomics"
Computational and Structural Biotechnology Journal, 4, e20131011. (2013) - 7.Kojima, M., Kamada-Nobusada, T., Komatsu, H., Takei, K., Kuroha, T., Mizutani, M., Ashikari, M., Ueguchi-Tanaka, M., Matsuoka, M., Suzuki, K. and Sakakibara, H.:
"Highly-sensitive and High-throughput analysis of plant hormones using MS-probe modification and liquid chromatography-tandem mass spectrometry: an application for hormone profiling in Oryza sativa"
Plant Cell Physiology, 50, 1201-1214 (2009)
Related Links
Lab Members
Principal investigator
- Masami Hirai
- Unit Leader
Core members
- Kiminori Toyooka
- Senior Technical Scientist
- Miki Fujita
- Senior Technical Scientist
- Mayuko Sato
- Technical Scientist
- Mikiko Kojima
- Expert Technician
- Ryosuke Sasaki
- Expert Technician
- Tetsuya Mori
- Expert Technician
- Makoto Kobayashi
- Technical Staff I
- Muneo Sato
- Technical Staff I
- Yumiko Takebayashi
- Technical Staff I
- Yumi Goto
- Technical Staff II
- Kouji Takano
- Technical Staff II
- Noriko Takeda
- Technical Staff II
- Mayumi Wakazaki
- Technical Staff II
- Akira Oikawa
- Senior Visiting Scientist
- Miyako Kusano
- Senior Visiting Scientist
- Hitoshi Sakakibara
- Senior Visiting Scientist
- Tetsuya Sakurai
- Senior Visiting Scientist
- Mitsunori Seo
- Senior Visiting Scientist
Contact Information
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Email: masami.hirai [at] riken.jp