RIKEN Center for Integrative Medical Sciences Laboratory for Retrotransposon Dynamics

Team Leader: Tomoichiro Miyoshi (Ph.D.)

Japanese Page

Research Summary

Retrotransposons are driving forces in genome evolution; however, their activities can cause genetic instability that leads to various diseases. Our group focuses on (1) the dynamic regulation of how mammalian retrotransposons mobilize in our genome, and (2) the mechanisms of aging and immune responses that retrotransposons facilitate. In the future, we aim to establish techniques to control the retrotransposon activities, thereby developing methods to suppress disease-associated phenotypes induced by retrotransposons.

Main Research Fields

Biological Sciences

Related Research Fields

Biology
Medicine, Dentistry & Pharmacy
Genome biology
Genetics
Molecular biology

Keywords

Genome
Retrotransposon
DNA repair
Senescence
Innate immunity

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

1. *Luqman-Fatah A., Watanabe Y., Ishikawa F., Moran J.V., and Miyoshi T.:
"The interferon stimulated gene-encoded protein HELZ2 inhibits human LINE-1 retrotransposition and LINE-1 RNA-mediated type I interferon induction."
bioRxiv. doi: https://doi.org/10.1101/2022.03.26.485892 (2022).
2. *Yamamoto I., Nakaoka H., Takikawa M., Tashiro S., Kanoh J., Miyoshi T., and Ishikawa F.:
"Fission yeast Stn1 maintains stability of repetitive DNA at subtelomere and ribosomal DNA regions."
Nucleic Acids Res. 49: 10465-10476 (2021).
3. *Miyoshi T., Makino T., and Moran J.V.:
"Poly(ADP-ribose) polymerase 2 recruits replication protein A to sites of LINE-1 integration to facilitate retrotransposition."
Molecular Cell. 75: 1286-1298 (2019).
4. *Kopera H.C., Flasch D.A., Nakamura M., Miyoshi T., Doucet A.J., and Moran J.V.:
"LEAP: L1 Element Amplification Protocol."
Methods Mol. Biol. 1400: 339-355 (2016).
5. *Takemata N., Oda A., Yamada T., Galipon J., Miyoshi T., Suzuki Y., Sugano S., Hoffman C.S., Hirota K., and Ohta K.:
"Local potentiation of stress-responsive genes by upstream noncoding transcription."
Nucleic Acids Res. 44: 5174-5189 (2016).
6. *Doucet A.J., Wilusz J.E., Miyoshi T., Liu Y., and Moran J.V.:
"A 3’ poly(A) tract is required for LINE-1 retrotransposition."
Molecular Cell. 60: 728-741 (2015).
7. *Miyoshi T., Ito M., and Ohta K.:
"Spatiotemporal regulation of meiotic recombination by Liaisonin."
Bioarchitecture. 3: 20-24 (2012)
8. *Miyoshi T., Ito M., Kugou K., Yamada S., Furuichi M., Oda A., Yamada T., Hirota K., Masai H., and Ohta K.:
"A central coupler for recombination initiation linking chromosome architecture to S-phase checkpoint."
Molecular Cell. 47: 722-733 (2012).
9. *Hirota K., Miyoshi T., Kugou K., Hoffman C.S., Shibata T., and Ohta K.:
"Stepwise chromatin remodelling by a cascade of transcription initiation of non-coding RNAs."
Nature. 456, 130-134 (2009).
10. *Miyoshi T., Kanoh J., Saito M., and Ishikawa F.:
"Fission yeast Pot1-Tpp1 protects telomeres and regulates telomere length."
Science. 320: 1341-1344 (2008).

Lab Members

Principal investigator

Tomoichiro Miyoshi: Team Leader

Contact Information

East Research Bld. 4F,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama, Kanagawa,
230-0045, Japan
Email: tomoichiro.miyoshi [at] riken.jp