Research Summary

Research Subjects

• Advancement of technologies in reproductive biology
• In vitro development of early mouse embryos

Selected Publications

• 1. Goto, T., Hagihara, M., Irie, S., Abe, T., Kiyonari, H., Miyamichi, K.
  "Dietary Availability Acutely Influences Puberty Onset via a Hypothalamic Neural Circuit."
  Neuron 2025
• 2. Wang, Y., Cao, S., Tone, D., Fujishima, H., Yamada, R.G., Ohno, R.I., Shi, S., Matsuzawa, K., Yada, S., Kaneko, M., Sakamoto, H., Onishi, T., Ukai-Tadenuma, M., Ukai, H., Hanashima, C., Hirose, K., Kiyonari, H., Sumiyama, K., Ode, K.L., Ueda, H.R.
  "Postsynaptic competition between calcineurin and PKA regulates mammalian sleep-wake cycles."
  Nature 2024 636(8042):412-421
• 3. Matsumoto, N., Miyano, M., Abe, T., Kashima, T., Kato-Ishikura, E., Inoue, KI., Liu, J., Kiyonari, H., Takeuchi, H., Ikegaya, Y.
  "Generation of Dopamine Transporter (DAT)-mCherry Knock-in Rats by CRISPR-Cas9 Genome Editing."
  Biol Pharm Bull 2024 47(2):394-398
• 4. Abe, T., kaneko, M., Kiyonari, H.
  "A reverse genetic approach in geckos with the CRISPR/Cas9 system by oocyte microinjection."
  Dev Biol 2023 497:26-32
• 5. Suita, K., Ishikawa, K., Kaneko, M., Wataki, A., Takahashi, M., Kiyonari, H., Sunagawa, G.A.
  "Mouse embryonic stem cells embody organismal-level cold resistance."
  Cell Rep 2023 42: 112954
• 6. Bilgic, M., Wu, Q., Suetsugu, T., Shitamukai, A., Tsunekawa, Y., Shimogori, T., Kadota, M., Nishimura, O., Kuraku, S., Kiyonari, H., Matsuzaki, F.
  "Truncated radial glia as a common precursor in the late corticogenesis of gyrencephalic mammals."
  eLife 2023 12:RP91406
• 7. Nishiyama, C., Saito, Y., Sakaguchi, A., Kaneko, M., Kiyonari, H., Xu, Y., Arima, Y., Uosaki, H., Kimura, W.
  "Prolonged Myocardial Regenerative Capacity in Neonatal Opossum."
  Circulation 2022 146: 125-139
• 8. Kiyonari, H., Kaneko, M., Abe, T., Shiraishi, A., Yoshimi, R., Inoue, K. I., Furuta, Y.
  "Targeted gene disruption in a marsupial, Monodelphis domestica, by CRISPR/Cas9 genome editing."
  Current Biology 2021 31, 3956-3963 e3954.
• 9. Abe, T., Inoue, K. I., Furuta, Y., Kiyonari, H.
  "Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes."
  Cell Reports 2020 31(7): 107653.
• 10. Kiyonari, H., Kaneko, M., Abe, T., Shioi, G., Furuta, Y., Fujimori T.
  "Dynamic organelle localization and cytoskeletal reorganization during preimplantation mouse embryo development revealed by live imaging of genetically encoded fluorescent fusion proteins."
  Genesis 2019 57(2):e23277.

Recent Research Results

• 

  Jul. 22, 2021
  Genome editing meets marsupials

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