RIKEN Center for Biosystems Dynamics Research Laboratory for Comprehensive Bioimaging
Team Director: Tomonobu Watanabe (Ph.D.)
Research Summary
Cells can be thought of as complex systems in a dynamic equilibrium between multiple states that fluctuate stochastically. Our aim is to construct a numerical model that describes the common mechanisms regulating high-order function in complex biological systems. To understand the behavior of these systems, it is necessary to observe state transitions while at the same time quantitatively describing their dynamics. We are developing novel imaging systems that combine three major techniques to achieve this aim: 1) imaging systems which measure protein position, dynamics and the surrounding environment , 2) novel cell culture systems which can control and perturb the chemical/mechanical environment, and 3) custom designed biochemical/inorganic sensors that can visualize the status of the cell. The developed techniques will provide significant insight on the regulation of tissues, immunity, metabolism and aging by understanding the fundamental mechanisms that give rise to these disparate and complex systems. In turn, the information gained will significantly improve our ability to diagnose and treat diseases and advance the design of new medicines.
Keywords
- Quantitative measurement
- Optical microscope
- Fluorescent protein
Selected Publications
- 1.
Fujita H, Haruki T, Sudo K, Koga Y, Nakamura Y, Abe K, Yoshida Y, Koizumi K, M Watanabe TM.:
"Yuragi biomarker concept for evaluating human induced pluripotent stem cells using heterogeneity-based Raman finger-printing."
Biophysics and Physicobiology, 21, Supplemental (2024) - 2.
Watanabe TM, Ueda S, Ishida S, Shioi G, Kaneshiro J, Magari M.:
"Optical evaluation of internal damage to human hair based on second near-infrared window polarization microscopy."
Int J Cosmet Sci., 46 (6) 850-864 (2024) - 3.
Ichinose J, Oba K, Arase Y, Kaneshiro J, Tate SI, Watanabe TM.:
"Quantitative prediction of rice starch digestibility using Raman spectroscopy and multivariate calibration analysis."
Food Chemistry, 435 (2024) - 4.
Okamoto K, Fujita H, Okada Y, Shinkai S, Onami S, Abe K, Fujimoto K, Sasaki K, Shioi G, Watanabe TM.:
"Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance."
EMBO J., 42 (18), (2023) - 5.
Fujita H, Kaneshiro J, Takeda M, Sasaki K, Yamamoto R, Umetsu D, Kuranaga E, Higo S, Kondo T, Asano Y, Sakata Y, Miyagawa S, Watanabe TM.:
"Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generation."
Life Sci Alliance, 6 (7) (2023) - 6.
Germond A, Panina Y, Shiga M, Niioka H, Watanabe TM.:
"Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy."
Anal Chem., 92 (22), (2020) - 7.
David BG, Fujita H, Yasuda K, Okamoto K, Panina Y, Ichinose J, Sato O, Horie M, Ichimura T, Okada Y, Watanabe TM.:
"Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells."
Stem Cell Res., 41 (2019) - 8.
Germond A, Ichimura T, Horinouchi T, Fujita H, Furusawa C, Watanabe TM.:
"Raman spectral signature reflects transcriptomic features of antibiotic resistance in Escherichia coli."
Commun Biol., 1:85 (2018) - 9.
Okamoto K, Germond A, Fujita H, Furusawa C, Okada Y, Watanabe TM.:
"Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation."
Sci Rep., 8 (1), (2018) - 10.
Ichimura T, Chiu LD, Fujita K, Kawata S, Watanabe TM, Yanagida T, Fujita H.:
"Visualizing cell state transition using Raman spectroscopy."
PLoS One., 9 (1), (2014)
Recent Research Results
Related Links
Lab Members
Principal investigator
- Tomonobu Watanabe
- Team Director
Core members
- Yasuhiro Maeda
- Technical Scientist
- Go Shioi
- Technical Scientist
- Junya Ichinose
- Research Scientist
- Michiko Inoue
- Technical Staff I
- Yuki Tano
- Assistant
Contact Information
Email: tomowatanabe@riken.jp