Research Summary

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

• 1. Kaneko S, Imai S, Asao N, Kofuku Y, Ueda T, Shimada I.:
  "Activation mechanism of the μ-opioid receptor by an allosteric modulator"
  Proc Natl Acad Sci U S A. 119(16), e2121918119. (2022) doi: 10.1073/pnas.2121918119.
• 2. Shiraishi Y, Kofuku Y, Ueda T, Pandey S, Dwivedi-Agnihotri H, Shukla AK, Shimada I.:
  "Biphasic activation of β-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR"
  Nature Communnications 2(1), 7158. (2021) doi: 10.1038/s41467-021-27482-3.
• 3. Iwahashi Y, Toyama Y, Imai S, Itoh H, Osawa M, Inoue M, Shimada I.:
  "Conformational equilibrium shift underlies altered K⁺ channel gating as revealed by NMR"
  Nature Communications 11, 5168 (2020) doi: 10.1038/s41467-020-19005-3
• 4. Mizukoshi Y, Takeuchi K, Tokunaga Y, Matsuo H, Imai M, Fujisaki M, Kamoshida H, Takizawa T, Hanzawa H, Shimada I.:
  "Targeting the cryptic sites: NMR-based strategy to improve protein druggability by controlling the conformational equilibrium"
  Science Advances 6(40), eabd0480 (2020) doi: 10.1126/sciadv.abd0480
• 5. Zhao Q, Fujimiya R, Kubo S, Marshall CB, Ikura M, Shimada I, Nishida N.:
  "Real-Time In-Cell NMR Reveals the Intracellular Modulation of GTP-Bound Levels of RAS"
  Cell Reports 32(8), 108074 (2020) doi: 10.1016/j.celrep.2020.108074
• 6. * Mizumura T, Kondo K, Kurita M, Kofuku Y, Natsume M, Imai S, Shiraishi Y, Ueda T, and Shimada I^*.:
  "Activation of adenosine A_2A receptor by lipids from docosahexaenoic acid revealed by NMR"
  Sci. Adv. (2020) doi: 10.1126/sciadv.eaay8544
• 7. * Imai S, Yokomizo T, Kofuku Y, Shiraishi Y, Ueda T, and Shimada I^*.:
  "Structural equilibrium underlying ligand -dependent activation of β₂ -adreno receptor."
  Nat. Chem. Bio. (2020) doi: 10.1038/s41589-019-0457-5.
• 8. * Kano H, Toyama Y, Imai S, Iwahashi Y, Mase Y, Yokogawa M, Osawa M, and Shimada I^*.:
  "Structural mechanism underlying G protein family-specific regulation of G protein-gated inwardly rectifying potassium channel"
  Nat. Commun. 10(1),2008. (2019)
• 9. * Shimada I^*, Ueda T, Kofuku Y, Matthew T. Eddy, and Kurt Wuthrich^*.:
  "GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures."
  Nat. Rev. Drug Discov. 18(1), 59-82 (2019)
• 10. * Shiraishi Y, Natsume M, Kofuku Y, Imai S, Nakata K, Mizukoshi T, Ueda T, Iwai H, and Shimada I^*.:
  "Phosphorylation-induced conformation of β₂-adrenoceptor related to the arrestin recruitment revealed by NMR"
  Nat. Commun. 9(1):194 (2018). doi: 10.1038/s41467-017-02632-8

Recent Research Results

• 

  Mar. 15, 2022
  Two-part tango triggers signal activation of key regulatory protein

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