Research Summary

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

• 1.Shunsuke Imai, Hiroshi Suzuki, Yoshinori Fujiyoshi, Ichio Shimada.:
  "Dynamically regulated two-site interaction of viral RNA to capture host translation initiation factor"
  Nature communications 14:4977 (2023). doi: 10.1038/s41467-023-40582-6
• 2.Yutaro Shiraishi, Ichio Shimada.:
  "NMR Characterization of the Papain-like Protease from SARS-CoV-2 Identifies the Conformational Heterogeneity in Its Inhibitor-Binding Site"
  Journal of the American Chemical Society 145:16669-16677 (2023). doi: 10.1021/jacs.3c04115
• 3.Kaneko S, Imai S, Asao N, Kofuku Y, Ueda T, Shimada I.:
  "Activation mechanism of the μ-opioid receptor by an allosteric modulator"
  Proc Natl Acad Sci U S A. 119(16), e2121918119. (2022) doi: 10.1073/pnas.2121918119.
• 4.Shiraishi Y, Kofuku Y, Ueda T, Pandey S, Dwivedi-Agnihotri H, Shukla AK, Shimada I.:
  "Biphasic activation of β-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR"
  Nature Communnications 2(1), 7158. (2021) doi: 10.1038/s41467-021-27482-3.
• 5.Iwahashi Y, Toyama Y, Imai S, Itoh H, Osawa M, Inoue M, Shimada I.:
  "Conformational equilibrium shift underlies altered K⁺ channel gating as revealed by NMR"
  Nature Communications 11, 5168 (2020) doi: 10.1038/s41467-020-19005-3
• 6.Mizukoshi Y, Takeuchi K, Tokunaga Y, Matsuo H, Imai M, Fujisaki M, Kamoshida H, Takizawa T, Hanzawa H, Shimada I.:
  "Targeting the cryptic sites: NMR-based strategy to improve protein druggability by controlling the conformational equilibrium"
  Science Advances 6(40), eabd0480 (2020) doi: 10.1126/sciadv.abd0480
• 7.* Mizumura T, Kondo K, Kurita M, Kofuku Y, Natsume M, Imai S, Shiraishi Y, Ueda T, and Shimada I^*.:
  "Activation of adenosine A_2A receptor by lipids from docosahexaenoic acid revealed by NMR"
  Sci. Adv. (2020) doi: 10.1126/sciadv.eaay8544
• 8.* Imai S, Yokomizo T, Kofuku Y, Shiraishi Y, Ueda T, and Shimada I^*.:
  "Structural equilibrium underlying ligand -dependent activation of β₂ -adreno receptor."
  Nat. Chem. Bio. (2020) doi: 10.1038/s41589-019-0457-5.
• 9.* Kano H, Toyama Y, Imai S, Iwahashi Y, Mase Y, Yokogawa M, Osawa M, and Shimada I^*.:
  "Structural mechanism underlying G protein family-specific regulation of G protein-gated inwardly rectifying potassium channel"
  Nat. Commun. 10(1),2008. (2019)
• 10.* Shimada I^*, Ueda T, Kofuku Y, Matthew T. Eddy, and Kurt Wuthrich^*.:
  "GPCR drug discovery: integrating solution NMR data with crystal and cryo-EM structures."
  Nat. Rev. Drug Discov. 18(1), 59-82 (2019)

Recent Research Results

• 

  Mar. 15, 2022
  Two-part tango triggers signal activation of key regulatory protein

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