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RIKEN Center for Biosystems Dynamics Research Laboratory for Translation Structural Biology

Team Leader: Takuhiro Ito (Ph.D.)

Research Summary

Takuhiro  Ito(Ph.D.)

We plan to conduct studies on three dimensional structures of the translation factors and/or the ribosome, mainly by X-ray crystallography and cryo-EM single particle analysis, focusing on the structural analysis of the large complexes that function in the eukaryotic translational system, in order to reveal the complicated mechanisms of eukaryotic translation.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Biology

Keywords

  • X-ray crystallography
  • NMR
  • Cryo-electron microscopy
  • translation

Selected Publications

  • 1. Zyryanova, AF., Kashiwagi, K., Rato, C., Harding, HP., Crespillo-Casado, A., Perera, LA., Sakamoto, A., Nishimoto, M., Yonemochi, M., Shirouzu, M., Ito, T., Ron, D.:
    "ISRIB blunts the integrated stress response by allosterically antagonising the inhibitory effect of phosphorylated eIF2 on eIF2B"
    Molecular Cell 81. 88-103, (2021) doi: 10.1016/j.molcel.2020.10.031
  • 2. Yokoyama, T., Machida, K., Iwasaki, W., Shigeta, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Harada, Y., Shigematsu, H., Shirouzu, M., Tadakuma, H., Imataka, H., Ito, T.:
    "HCV IRES Captures an Actively Translating 80S Ribosome"
    Molecular cell. 74 1205-1214. (2019) doi: 10.1016/j.molcel.2019.04.022
  • 3. Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T.:
    "Structural basis for eIF2B inhibition in integrated stress response"
    Science. 364 495-499. (2019) doi: 10.1126/science.aaw4104
  • 4. Iwasaki, S., Iwasaki, W., Takahashi, M., Sakamoto, A., Watanabe, C., Shichino, Y., Floor, S.N., Fujiwara, K., Mito, M., Dodo, K., Sodeoka, M., Imataka, H., Honma, T., Fukuzawa, K., Ito, T., Ingolia, N.T.:
    "The translation inhibitor Rocaglamide targets a bimolecular cavity between eIF4A and polypurine RNA"
    Molecular Cell. 73 738-748. (2018) doi: 10.1016/j.molcel.2018.11.026
  • 5. Kashiwagi, K., Takahashi, M., Nishimoto, M., Hiyama, T.B., Higo, T., Umehara, T., Sakamoto, K., Ito, T. and Yokoyama, S.:
    "Crystal structure of eukaryotic translation initiation factor 2B"
    Nature. 531 122-125. (2016) doi: 10.1038/nature16991
  • 6. Ito, T., Masuda, I., Yoshida, K., Goto-Ito, S., Sekine, S., Suh, S.W., Hou, Y.-M. and Yokoyama, S.:
    "Structural Basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD"
    Proc Natl Acad Sci U S A. 112 E4197-E4205. (2015) doi: 10.1073/pnas.1422981112
  • 7. Ito, T., Yokoyama, S.:
    "Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions"
    Nature 467. 612-616 (2010) doi: 10.1038/nature09411
  • 8. Goto-Ito, S., Ito, T., Kuratani, M., Bessho, Y., Yokoyama, S.:
    "Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation"
    Nature Structural & Molecular Biology 16. 1109-1115 (2009) doi: 10.1038/nsmb.1653

Recent Research Results

Related Links

Lab Members

Principal investigator

Takuhiro Ito
Team Leader

Core members

Wakana Iwasaki
Senior Research Scientist
Kazuhiro Kashiwagi
Research Scientist
Madoka Nishimoto
Technical Scientist
Mari Takahashi
Technical Scientist
Ayako Sakamoto
Technical Scientist

Contact Information

West Research Building,
1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa
230-0045, Japan
Tel: +81-(0)45-503-9204
Fax: +81-(0)45-503-9460
Email: takuhiro.ito [at] riken.jp

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