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RIKEN Center for Biosystems Dynamics Research Laboratory for Translation Structural Biology

Team Leader: Takuhiro Ito (Ph.D.)

Research Summary

Takuhiro  Ito(Ph.D.)

We plan to conduct studies on three dimensional structures of the translation factors and/or the ribosome, mainly by X-ray crystallography and cryo-EM single particle analysis, focusing on the structural analysis of the large complexes that function in the eukaryotic translational system, in order to reveal the complicated mechanisms of eukaryotic translation.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Biology

Keywords

  • X-ray crystallography
  • NMR
  • Cryo-electron microscopy
  • translation

Selected Publications

  • 1. Yokoyama, T., Machida, K., Iwasaki, W., Shigeta, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Harada, Y., Shigematsu, H., Shirouzu, M., Tadakuma, H., Imataka, H., Ito, T.:
    "HCV IRES Captures an Actively Translating 80S Ribosome"
    Molecular cell. 74 1205-1214. (2019).
  • 2. Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T.:
    "Structural basis for eIF2B inhibition in integrated stress response"
    Science. 364 495-499. (2019).
  • 3. Iwasaki, S., Iwasaki, W., Takahashi, M., Sakamoto, A., Watanabe, C., Shichino, Y., Floor, S.N., Fujiwara, K., Mito, M., Dodo, K., Sodeoka, M., Imataka, H., Honma, T., Fukuzawa, K., Ito, T., Ingolia, N.T.:
    "The translation inhibitor Rocaglamide targets a bimolecular cavity between eIF4A and polypurine RNA"
    Molecular Cell. 73 738-748. (2018).
  • 4. Kashiwagi, K., Takahashi, M., Nishimoto, M., Hiyama, T.B., Higo, T., Umehara, T., Sakamoto, K., Ito, T. and Yokoyama, S.:
    "Crystal structure of eukaryotic translation initiation factor 2B"
    Nature. 531 122-125. (2016).
  • 5. Kashiwagi, K., Shigeta, T., Imataka, H., Ito, T., Yokoyama, S.:
    "Expression, purification, and crystallization of Schizosaccharomyces pombe eIF2B"
    J Struct Funct Genomics. 17 33-38 (2016).
  • 6. Ito, T., Masuda, I., Yoshida, K., Goto-Ito, S., Sekine, S., Suh, S.W., Hou, Y.-M. and Yokoyama, S.:
    "Structural Basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD"
    Proc Natl Acad Sci U S A. 112 E4197-E4205. (2015).
  • 7. Kuwasako, K., Takahashi, M., Unzai, S., Tsuda, K., Yoshikswa, S., He, F., Kobayashi, N., Guntert, P., Shirouzu, M., Ito, T., Tanaka, A., Yokoyama, S., Hagiwara, M., Kuroyanagi, H. and Muto, Y.:
    "RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing"
    Nat Struct Mol Biol. 21 778-86. (2014).
  • 8.Kashiwagi, K., Ito, T. and Yokoyama, S.:
    "Crystal structure of the eukaryotic translation initiation factor 2A from Schizosaccharomyces pombe"
    J Struct Funct Genomics. 15 125-130. (2014).
  • 9.Kashiwagi, K., Ito, T. and Yokoyama, S.:
    "Crystal structure of the eukaryotic translation initiation factor 2A from Schizosaccharomyces pombe"
    J Struct Funct Genomics. 15 125-130. (2014).
  • 10.Kashiwagi, K., Ito, T. and Yokoyama, S.:
    "Crystal structure of the eukaryotic translation initiation factor 2A from Schizosaccharomyces pombe"
    J Struct Funct Genomics. 15 125-130. (2014).

Recent Research Results

Related Links

Lab Members

Principal investigator

Takuhiro Ito
Team Leader

Core members

Wakana Iwasaki
Senior Research Scientist
Kazuhiro Kashiwagi
Research Scientist
Madoka Nishimoto
Technical Scientist
Mari Takahashi
Technical Scientist
Ayako Sakamoto
Technical Scientist

Contact Information

West Research Building,
1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa
230-0045, Japan
Tel: +81-(0)45-503-9204
Fax: +81-(0)45-503-9460

Email: takuhiro.ito [at] riken.jp

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