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RIKEN BioResource Research Center Gene Engineering Division (RIKEN BRC Gene Bank)

Director: Yoshihiro Miwa (Ph.D.)

Research Summary

Genetic materials such as plasmids, clone sets of bacterial artificial chromosome (BAC), recombinant adenoviruses, expression vectors, and host bacteria are the most fundamental and essential research tools. They are used in the almost all fields of the life science, from basic researches to innovations. The Gene Engineering Division collects, conducts strict quality control of genetic materials and provides domestic and international scientific community with the materials of ensured reproducibility of experimental results. We aim to facilitate life science researches and innovations for improvement of human welfare and for solution of environmental problems and hope to contribute to the sustainable development of our race. Since 2002, the Division has been selected as a core facility of DNA materials by National BioResource Project (NBRP) funded by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan for collection, quality control, preservation and distribution of genetic materials of human, animal and microbe origin.

Research Subjects

  • Collection, preservation and distribution of genetic materials (DNA and cDNA).
  • Development of handling and preservation technique.
  • Development of quality control technique.
  • Development of application method of recombinant virus and its quality control.
  • Development of technology for medical sciences, biomass engineering and innovation.

Selected Publications

  • 1.Inagawa, K., Miyamoto, K., Yamakawa, H., Muraoka, N., Sadahiro, T., Umei, T., Wada, R., Katsumata, Y., Kaneda, R., Nakade, K., Kurihara, C., Obata, Y., Miyake, K., Fukuda, K. and Ieda, M.:
    "Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5."
    Circ. Res., 111, 1147-1156 (2012)
  • 2.Kawashima, R., Abei, M., Fukuda, K., Nakamura, K., Murata, T., Wakayama, M., Seo, E., Hasegawa, N., Mizuguchi, H., Obata, Y., Hyodo, I., Hamada, H. and Yokoyama, K.:
    "EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus."
    Inter. J. Cancer, 129, 1244-1253 (2011)
  • 3.Masuya, H., Makita, Y., Kobayashi, N., Nishikata, K., Yoshida, Y., Mochizuki, Y., Doi, K., Takatsuki, T., Waki, K., Tanaka, N., Ishii, M., Matsushima, A., Takahashi, S., Hijikata, A., Kozaki, K., Furuichi, T., Kawaji, H., Wakana, S., Nakamura, Y., Yoshiki, A., Murata, T., Fukami, K., Sujatha, M. S., Ohara, O., Hayashizaki, Y., Obata, Y., Toyoda, T.:
    "The RIKEN integrated database of mammals."
    Nucleic Acids Research, 39, D861-D870 (2011)
  • 4.Pan, J., Nakade, K., Huang, Y-C., Zhu, Z-W., Masuzaki, S., Hasegawa, H., Murata, T., Yoshiki, A., Yamaguchi, N., Lee, C-H., Yang, W-C., Tsai, E-M., Obata, Y. and Yokoyama, K.:
    "Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2."
    Oncogene, 29, 6245-6256 (2010)
  • 5.Fukuda, K., Abei, M., Ugai, H., Kawashima, R., Seo, E., Wakayama, M., Murata, T., Endo, S., Hamada, H., Hyodo, I., and Yokoyama, K.:
    "E1A, E1B double-restricted replicative adenovirus at low dose greatly augments tumor-specific suicide gene therapy for gallbladder cancer."
    Cancer Gene Therapy, 16, 126-136 (2009)
  • 6.Nakade, K., Pan, J., Yamasaki, T., Murata, T., Wasylyk, B. and Yokoyama, K.:
    "JDP2 (Jun dimerization protein 2)-deficient mouse embryonic fibroblasts are resistant to replicative senescence."
    J. Biol. Chem., 284, 10808-10817 (2009)
  • 7.Yamasaki, T., Takahashi, A., Pan. J., Yamaguchi. N. and Yokoyama, K.:
    "Phosphorylation of activation transcription factor-2 (ATF-2) at serine 121 by protein kinase C controls c-Jun-mediated activation of transcription."
    J. Biol. Chem., in press (2009)
  • 8.Hirano, T., Ike, F., Murata, T., Obata, Y., Utiyama, H. and Yokoyama, K.:
    "Genes encoded within 8q24 on the amplicon of a large extrachromosomal element are selectively repressed during the terminal differentiation of HL-60 cells."
    Mutation Research 640, 97-106 (2008)
  • 9.Murata, T., Shinozuka, Y., Obata, Y. and Yokoyama, K.:
    "Phosphorylation of two eukaryotic transcription factors, Jun dimerization protein 2 and activation transcription factor 2, in Escherichia coli by Jun N-terminal kinase 1."
    Anal. Biochem. 376, 115-121 (2008)
  • 10.Nakade, K., Pan, J., Yoshiki, A., Ugai, H., Kimura, M., Liu, B., Li, H., Obata, Y., Iwama, M., Itohara, S., Murata, T. and Yokoyama, K.:
    "JDP2 suppresses adipocyte differentiation by regulating histone acetylation."
    Cell Death and Differentiaton 14, 1398-1405 (2007)

Related Links

Lab Members

Principal investigator

Yoshihiro Miwa

Core members

Takehide Murata
Senior Research Scientist
Koji Nakade
Research & Development Scientist
Shotaro Kishikawa
Technical Scientist
Yukari Kujime
Technical Staff II
Chitose Kurihara
Technical Staff II
Megumi Sakayori
Technical Staff II
Satoko Masuzaki
Technical Staff II
Nobuatsu Nakamura
Technical Staff II
Kenichi Nakashima
Technical Staff II
Yukiko Tanigawa
Technical Staff II
Syunichi Sasanuma
Technical Staff II

Contact Information

3-1-1 Koyadai
Tsukuba, Ibaraki
305-0074 Japan