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RIKEN BioResource Research Center (BRC®) Gene Engineering Division (RIKEN DNA Bank)

Director: Yoshihiro Miwa (Ph.D.)

Research Summary

 Yoshihiro Miwa (Ph.D.)

Genetic materials including plasmids, such as expression vector and imaging tools, clone sets of human full-length cDNA clone and BAC, recombinant adenoviruses are the most fundamental and essential research tools. They are used in any fields of the life science, from basic researches to innovations. The Gene Engineering Division collects, conducts strict quality control, such as whole sequencing, of plasmid of genetic materials and provides domestic and international scientific community with the materials of ensured reproducibility of experimental results. We aim to facilitate life science researches and innovations for improvement of human welfare and for solution of environmental problems and hope to contribute to the sustainable development of our race. Since 2002, the Division has been fulfilling its responsibility to develop DNA materials in Japan as a core facility of the National BioResource Project (NBRP).

Research Subjects:

  • Collection, development, preservation and distribution of genetic materials (DNA and cDNA).
  • Development of quality control, handling and preservation technique.
  • Development of application method of recombinant virus and its quality control.
  • Development of technology for medical sciences and innovation.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Agricultural Sciences
  • Complex Systems
  • Biology
  • Medicine, Dentistry & Pharmacy

Keywords

  • plasmid
  • genetic tool
  • human full-length cDNA
  • virus
  • imaging

Selected Publications

  • 1.Mizuno-Iijima S, Kawamoto S, Asano M, Mashimo T, Wakana S,Nakamura K, Nishijima K, Okamoto H, Saito K, Yoshina S, Miwa Y,Nakamura Y, Ohkuma M, Yoshiki A.:
    "Mammalian genome research resources available from the National BioResource Project in Japan."
    Mammalian Genome 10.1007/s00335-024-10063-2 (2024)
  • 2.Nakade K, Tsukamoto S, Nakashima K, An Y, Sato I, Li J, Hemmi Y,Miwa Y, Hayashi Y.:
    "Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system."
    Cells Rep. Methods 3, 100662 (2023)
  • 3.Tsukamoto S, Nakade K, Wakabayashi T, Nakashima K, Takami M,Henmi Y, Kuramochi Y, Shimizu T, Arai Y, Takasaki M, Noguchi M,Nakamura Y, Miwa Y, Hayashi Y.:
    "Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing"
    Stem Cell Res 53 102363 doi:10.1016/j.scr.2021.102363 (2021)
  • 4.Kulathunga, K., Wakimoto, A., Hiraishi, Y., Yadav, MK., Gentleman, K., Warabi, E., Sakasai, T., Miwa, Y., Mizuno, S., Takahashi, S., Hamada, M.:
    "Albino mice with the point mutation at the tyrosinase locus show high cholesterol diet-induced NASH susceptibility."
    SCIENTIFIC REPORTS 11:21827 doi: 10.1038/s41598-021-00501-5 (2021).
  • 5.Mizuno-Iijima, S., Ayabe, S., Kato, K., Matoba, S., Ikeda, Y., Dinh, TTH., Le, HT., Suzuki, H., Nakashima, K., Hasegawa, Y., Hamada, Y., Tanimoto, Y., Daitoku, Y., Iki, N., Ishida, M., Ibrahim, EAE., Nakashiba, T., Hamada, M., Murata, K., Miwa, Y., Okada-Iwabu, M., Iwabu, M., Yagami, K., Ogura, A., Obata, Y., Takahashi, S., Mizuno, S., Yoshiki, A., Sugiyama, F.:
    "Efficient production of large deletion and gene fragment knock-in mice mediated by genome editing with Cas9-mouse Cdt1 in mouse zygotes."
    Methods S1046-2023(19)30328-7 doi:10.1016/j.ymeth.2020.04.007 (2020).
  • 6.Ayabe, S., Nakashima, K., Yoshiki, A.:
    "Off- and on-target effects of genome editing in mouse embryos."
    J Reprod Dev 65 1-5 (2019).
  • 7.Kurihara, C., Nakade, K., Pan, J., Huang, J., Wasylyk, B., Obata, Y.:
    "An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes"
    Biotechnol Rep Vol.12 26-32 (2016).
  • 8.Kohara, K., Pignatelli, M., Rivest, AJ., Jung, HY., Kitamura, T., Suh, J., Frank, D., Kajikawa, K., Mise, N., Obata, Y., Wickersham, IR., Tonegawa, S.:
    "Cell type-specific genetic and optogenetic tools reveal hippocampal CA2 circuits."
    Nat Neurosci Vol. 17 269-279 (2014).
  • 9.Hasegawa, N., Abei, M., Yokoyama, K., Fukuda, K., Seo, E., Kawashima, R., Nakano, Y., Yamada, T., Nakade, K., Hamada, H., Obata, Y., and Hyodo, I.:
    "Cyclophosphamide enhances antitumor efficacy of oncolytic adenovirus expressing uracil phosphoribosyltransferase (UPRT) in immunocompetent Syrian hamsters."
    Int. J. Cancer, 133, 1479-1488 (2013).
  • 10.Masuya, H., Makita, Y., Kobayashi, N., Nishikata, K., Yoshida, Y., Mochizuki, Y., Doi, K., Takatsuki, T., Waki, K., Tanaka, N., Ishii, M., Matsushima, A., Takahashi, S., Hijikata, A., Kozaki, K., Furuichi, T., Kawaji, H., Wakana, S., Nakamura, Y., Yoshiki, A., Murata, T., Fukami, K., Sujatha, M. S., Ohara, O., Hayashizaki, Y., Obata, Y., Toyoda, T.:
    "The RIKEN integrated database of mammals."
    Nuc. Acids Res., 39, D861-D870 (2011).

Related Links

Lab Members

Principal investigator

Yoshihiro Miwa
Director

Core members

Takehide Murata
Senior Research Scientist
Tetsushi Iida
Research Scientist
Shotaro Kishikawa
Technical Scientist
Shingo Nozaki
Research & Development Scientist
Junichi Onami
Research & Development Scientist
Yukari Kujime
Technical Staff II
Megumi Sakayori
Technical Staff II
Nobuatsu Nakamura
Technical Staff II
Kenichi Nakashima
Technical Staff II
Syunichi Sasanuma
Technical Staff II
Junko Kijima
Technical Staff II

Contact Information

3-1-1 Koyadai
Tsukuba, Ibaraki
305-0074 Japan
Email: dnabank.brc@riken.jp

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