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Chief Scientist Laboratories Chromosome Dynamics Laboratory

Chief Scientist: Tatsuya Hirano (Ph.D.)

Research Summary

Tatsuya  Hirano(Ph.D.)

The duplication and segregation of the genome are two of the most fundamental events in cell reproduction. Our laboratory is interested in understanding the molecular mechanisms that regulate the faithful segregation of chromosomes during mitosis. By using a cell-free extract derived from Xenopus laevis (African toad) eggs, we have discovered two multiprotein complexes, condensin and cohesin, that play central roles in chromosome condensation and sister chromatid cohesion, respectively. At the heart of the two complexes lie members of a large family of chromosomal ATPases, the SMC (structural maintenance of chromosomes) family. Accumulating lines of evidence suggest that SMC proteins actively participate in many aspects of higher-order chromosome dynamics, including chromosome-wide gene regulation, DNA recombination /repair and meiotic chromosome segregation. The long-term goal in our laboratory is to understand how condensin and cohesin work at a mechanistic level in vitro, and how they interact with other proteins to execute their essential functions in vivo. We take multidisciplinary approaches toward this goal, including biochemistry, cell biology, structural biology and biophysics. Our research will contribute to a better understanding of human health because chromosome anomalies, such as aneuploidy and translocations, are tightly associated with tumor development and birth defects.

Main Research Fields

  • Biology & Biochemistry

Related Research Fields

  • Molecular Biology & Genetics

Research Subjects

  • Chromosome architecture and segregation
  • Molecular mechanisms of action of SMC protein complexes
  • Chromosome dynamics and human disease

Selected Publications

  • 1.Yoshida, M. M., Kinoshita, K., Shintomi, K., Aizawa, Y., Hirano, T.:
    "Regulation of condensin II by self-suppression and release mechanisms."
    Mol. Biol. Cell, 35(2):ar21 (2024).
  • 2. Tane, S., Shintomi, K., Kinoshita, K., Tsubota, Y., Yoshida, M. M., Nishiyama, T., and Hirano, T.:
    "Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit."
    eLife 11:e84694 (2022).
  • 3. Yoshida, M. M., Kinoshita, K., Aizawa, Y., Tane, S., Yamashita, D., Shintomi, K., and Hirano, T.:
    "Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays."
    eLife 11:e78984 (2022).
  • 4. Kinoshita, K., Tsubota, Y., Tane, S., Aizawa, Y., Sakata, R., Takeuchi, K., Shintomi, K., Nishiyama, T., and Hirano, T.:
    "A loop extrusion-independent mechanism contributes to condensin I-mediated chromosome shaping."
    J. Cell Biol. 221:e202109016 (2022).
  • 5. Shintomi, K., Hirano, T.:
    "Guiding functions of the C-terminal domain of topoisomerase IIα advance mitotic chromosome assembly."
    Nat Commun 12, 2917 (2021).
  • 6. Shintomi, K., Inoue, F., Watanabe, H., Ohsumi, K., Ohsugi, M., and Hirano, T.:
    "Mitotic chromosome assembly despite nucleosome depletion in Xenopus egg extracts."
    Science 356, 1284-1287 (2017).
  • 7. Ono, T., Sakamoto, C., Nakao, M., Saitoh, N., and Hirano, T.:
    "Condensin II plays an essential role in reversible assembly of mitotic chromosomes in situ."
    Mol. Biol. Cell. 28, 2875-2886 (2017).
  • 8. Hirano, T.:
    "Condensin-based chromosome organization from bacteria to vertebrates."
    Cell 164, 847-857 (2016).
  • 9. Shintomi, K., Takahashi, T. S., and Hirano, T.:
    "Reconstitution of mitotic chromatids with a minimum set of purified factors."
    Nat. Cell Biol. 17, 1014-1023 (2015).
  • 10. Kinoshita, K., Kobayashi, T. J., and Hirano, T.:
    "Balancing acts of two HEAT subunits of condensin I support dynamic assembly of chromosome axes."
    Dev. Cell 33, 94-106 (2015).

Recent Research Results

Annual research report

Related Links

Lab Members

Principal investigator

Tatsuya Hirano
Chief Scientist

Core members

Takao Ono
Senior Research Scientist
Kazuhisa Kinoshita
Senior Research Scientist
Keishi Shintomi
Senior Research Scientist
Toshiharu Fujita
Postdoctoral Researcher
Yuki Masahara
Technical Staff I
Yuuki Aizawa
Technical Staff II
Tomoko Maruyama

Contact Information

2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Email: hiranot [at] riken.jp