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RIKEN Center for Sustainable Resource Science Plant Symbiosis Research Team

Team Leader: Makoto Hayashi (Ph.D.)

Research Summary

Makoto  Hayashi(Ph.D.)

Nitrogen is the most heavily used fertilizer in the present agriculture. Its production and use however damage the ecosystem due to the emission of greenhouse gases. Soil bacteria called rhizobia infect legume roots, and fix atmospheric nitrogen in root nodules. Consequently, if cereals such as rice, corn and wheat establish symbiosis with rhizobia, we can dramatically reduce the use of nitrogen fertilizer, resulted in ecosystem-friendly, sustainable agriculture. In order to achieve our goal, we aim to confer the ability to fix nitrogen on cereals, by elucidating molecular-genetic and biochemical functions of nodulation and symbiotic nitrogen fixation, as well as by investigating evolutionary aspects of legume-rhizobia symbiosis.

Main Research Fields

  • Biology

Related Research Fields

  • Agricultural Sciences


  • Plant-microbe interaction/Symbiosis
  • Biological interaction

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1.*Małolepszy A, Mun T, Sandal N, Gupta V, Dubin M, Urbański D, Shah N, Bachmann A, Fukai E, Hirakawa H, Tabata S, Nadzieja M, Markmann K, Su J, Umehara Y, Soyano T, Miyahara A, Sato S, Hayashi M, Stougaard J, Andersen SU:
    "The LORE1 insertion mutant resource"
    Plant J. 88: 306-317 (2016).
  • 2.Yamazaki A, Hayashi M:
    "Building the interaction interfaces: host responses upon infection with microorganisms"
    Curr. Opin. Plant Biol. 23: 132-139 (2015).
  • 3.*Soyano T, Shimoda Y, Hayashi M:
    "NODULE INCEPTION Antagonistically Regulates Gene Expression with Nitrate in Lotus japonicas"
    Plant Cell Physiol. 56: 368-376 (2015).
  • 4.*Soyano T, Hirakawa H, Sato S, Hayashi M, Kawaguchi M:
    "NODULE INCEPTION creates a long-distance negative feedback loop involved in homeostatic regulation of nodule organ production"
    Proc. Natl. Acad. Sci. USA 111: 14607-14612 (2014).
  • 5.*Soyano T, Hayashi M:
    "Transcriptional networks leading to symbiotic nodule organogenesis"
    Curr. Opin. Plant Biol. 20: 146-154 (2014).
  • 6.*Hayashi T, Shimoda Y, Sato S, Tabata S, Imaizumi-Anraku H, Hayashi M:
    "Rhizobial infection does not require the cortical expression of upstream common symbiosis genes responsible for the induction of Ca2+ spiking"
    Plant J. 77: 146-159 (2014).
  • 7.*Soyano T, Kouchi H, Hirota A, Hayashi M:
    "NODULE INCEPTION directly targets NF-Y subunit genes to regulate essential processes of root nodule development in Lotus japonicas"
    PLoS Genet. 9: e1003352 (2013).
  • 8.*Takeda N, Maekawa T, Hayashi M:
    "Nuclear-localized and deregulated calcium- and calmodulin-dependent protein kinase activates rhizobial and mycorrhizal responses in Lotus japonicas"
    Plant Cell 24: 810-822 (2012).
  • 9.*Shimoda Y, Han L, Yamazaki T, Suzuki R, Hayashi M, Imaizumi-Anraku H:
    "Rhizobial and fungal symbioses show different requirements for calmodulin binding to calcium calmodulin–dependent protein kinase in Lotus japonicas"
    Plant Cell 24: 304-321 (2012).
  • 10.*Fukai E, Soyano T, Umehara Y, Nakayama S, Hirakawa H, Tabata S, Sato S, Hayashi M:
    "Establishment of a Lotus japonicus gene tagging population using the exon-targeting endogenous retrotransposon LORE1"
    Plant J. 69: 720-730 (2012).

Recent Research Results

Related Links

Lab Members

Principal investigator

Makoto Hayashi
Team Leader

Core members

Tsuneo Hakoyama
Research Scientist
Akihiro Yamazaki
Research Scientist
Kai Battenberg / Kai Tsujii
Postdoctoral Researcher
Teruki Sugiyama
Postdoctoral Researcher
Atsuko Hirota
Technical Staff I
Shouko Yamazaki
Technical Staff II

Contact Information

1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Tel: +81-(0)45-503-9493
Fax: +81-(0)45-503-9492

Email: makoto.hayashi [at] riken.jp