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RIKEN Center for Sustainable Resource Science Biomolecular Characterization Unit

Unit Leader: Naoshi Dohmae (Ph.D.)

Research Summary

Naoshi  Dohmae(Ph.D.)

Our unit provides high quality structural characterization methods to the field of biological science, aiming to further understand the mechanism and action of biological molecules. We manage specialized and technical instruments including protein chemical analyses, mass spectrometry. Our challenge to research, develop and fine-tune novel characterization methods for biological molecules, is an endless yet rewarding process.

Main Research Fields

  • Biology

Related Research Fields

  • Environmental Science
  • Chemistry
  • Biological Sciences
  • Agricultural Sciences
  • Medicine, Dentistry & Pharmacy


  • Post-translational modofocations
  • Proteomics
  • Mass spectrometry
  • Epigenomics
  • Structural characterization

Selected Publications

  • 1.Saloura V, Vougiouklakis T, Zewde M, Deng X, Kiyotani K, Park JH, Matsuo Y, Lingen M, Suzuki T, Dohmae N, Hamamoto R, Nakamura Y
    "WHSC1L1-mediated EGFR mono-methylation enhances the cytoplasmic and nuclear oncogenic activity of EGFR in head and neck cancer."
    Sci Rep. 7:40664 (2017)
  • 2.Kwak HG, Dohmae N.:
    "Characterization of post-translational modifications on lysine 9 of histone H3 variants in mouse testis using matrix-assisted laser desorption/ionization-in source decay."
    Rapid Commun Mass Spectrom. 30(23): 2529–36 (2016)
  • 3.Shihoya W, Nishizawa T, Okuta A, Tani K, Dohmae N, Fujiyoshi Y, Nureki O, Doi T.:
    "Activation mechanism of endothelin ETB receptor by endothelin-1."
    Nature. 537(7620):363-68 (2016)
  • 4.Tsuchiya H, Doki S, Takemoto M, Ikuta T, Higuchi T, Fukui K, Usuda Y, Tabuchi E, Nagatoishi S, Tsumoto K, Nishizawa T, Ito K, Dohmae N, Ishitani R, Nureki O.:
    "Structural basis for amino acid export by DMT superfamily transporter YddG."
    Nature. 534:417–20 (2016)
  • 5.Yanagisawa T, Takahashi H, Suzuki T, Masuda A, Dohmae N, Yokoyama S.:
    "Neisseria meningitidis Translation Elongation Factor P and Its Active-Site Arginine Residue Are Essential for Cell Viability."
    PLoS ONE11, ;11(2):e0147907 (2016)
  • 6.Nakayama H, Yamauchi Y, Taoka M, Isobe T:
    "Direct identification of human cellular microRNAs by nanoflow liquid chromatography-high-resolution tandem mass spectrometry and database searching."
    Anal Chem. 87(5):2884-91 (2015)
  • 7.Huang C, Harada Y, Hosomi A, Masahara-Negishi Y, Seino J, Fujihira H, Funakoshi Y, Suzuki T, Dohmae N, Suzuki T.:
    "Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells."
    Proc Natl Acad Sci U S A112,1398-1403 (2015)
  • 8.Kumazaki K, Chiba S, Takemoto M, Furukawa A, Nishiyama K, Sugano Y, Mori T, Dohmae N, Hirata K, Nakada-Nakura Y, Maturana AD, Tanaka Y, Mori H, Sugita Y, Arisaka F, Ito K, Ishitani R, Tsukazaki T, Nureki O.:
    "Structural basis of Sec-independent membrane protein insertion by YidC."
    Nature509,516-520 (2014)
  • 9.Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O.:
    "Crystal structure of cas9 in complex with guide RNA and target DNA."
    Cell156,935-949 (2014)
  • 10.Masuda A, Dohmae N.:
    "Examination of an absolute quantity of less than a hundred nanograms of proteins by amino acid analysis."
    Anal Bioanal Chem405,8073-8081 (2013)

Recent Research Results

Related Links

Lab Members

Principal investigator

Naoshi Dohmae
Unit Leader

Core members

Hiroshi Nakayama
Senior Research Scientist
Kowashi Watanabe
Senior Technical Scientist
Takehiro Suzuki
Senior Technical Scientist
Ryousuke Matsuura
Junior Research Associate
Masami Koike
Technical Staff I
Hogeun Kwak
Technical Staff I

Contact Information

Bioscience Building,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan

Email: dohmae [at] riken.jp