RIKEN Center for Sustainable Resource Science Biomolecular Characterization Unit
Unit Leader: Naoshi Dohmae (Ph.D.)
Research Summary
Our unit provides high quality structural characterization methods to the field of biological science, aiming to further understand the mechanism and action of biological molecules. We manage specialized and technical instruments including protein chemical analyses, mass spectrometry. Our challenge to research, develop and fine-tune novel characterization methods for biological molecules, is an endless yet rewarding process.
Main Research Fields
- Biology
Related Research Fields
- Environmental Science
- Chemistry
- Biological Sciences
- Agricultural Sciences
- Medicine, Dentistry & Pharmacy
Keywords
- Post-translational modofocations
- Proteomics
- Mass spectrometry
- Epigenomics
- Structural characterization
Selected Publications
- 1.
Kato K, Shinoda T, Nagao R, Akimoto S, Suzuki T, Dohmae N, Chen M, Allakhverdiev SI, Shen JR, Akita F, Miyazaki N, Tomo T.:
"Structural basis for the adaptation and function of chlorophyll f in photosystem I."
Nat Commun. 11(1):238 (2020) - 2.
Nakayama H, Yamauchi Y, Nobe Y, Sato K, Takahashi N, Shalev-Benami M, Isobe T, Taoka M.:
"Method for Direct Mass-Spectrometry-Based Identification of Monomethylated RNA Nucleoside Positional Isomers and Its Application to the Analysis of Leishmania rRNA."
Anal Chem. 91(24):15634-15643 (2019) - 3.
Nagao R, Kato K, Suzuki T, Ifuku K, Uchiyama I, Kashino Y, Dohmae N, Akimoto S, Shen JR, Miyazaki N, Akita F.:
"Structural basis for energy harvesting and dissipation in a diatom PSII-FCPII supercomplex."
Nature Plants. 5:890-901 (2019) - 4.
Nagao R, Ueno Y, Akita F, Suzuki T, Dohmae N, Akimoto S, Shen JR.:
"Biochemical characterization of photosystem I complexes having different subunit compositions of fucoxanthin chlorophyll a/c-binding proteins in the diatom Chaetoceros gracilis."
Photosynth Res. 140(2):141-149 (2019) - 5.
Yoshida K, Muratani M, Araki H, Miura F, Suzuki T, Dohmae N, Katou Y, Shirahige K, Ito T, Ishii S.:
"Mapping of histone-binding sites in histone replacement-completed spermatozoa.s."
Nat Commun. 9(1):3885 (2018) - 6.
Kasuya G, Nakane T, Yokoyama T, Jia Y, Inoue M, Watanabe K, Nakamura R, Nishizawa T, Kusakizako T, Tsutsumi A, Yanagisawa H, Dohmae N, Hattori M, Ichijo H, Yan Z, Kikkawa M, Shirouzu M, Ishitani R, Nureki O.:
"Cryo-EM structures of the human volume-regulated anion channel LRRC8."
Nat Struct Mol Biol. 25(9):797-804 (2018) - 7.
Takahashi F, Suzuki T, Osakabe Y, Betsuyaku S, Kondo Y, Dohmae N, Fukuda H, Yamaguchi-Shinozaki K, Shinozaki K.:
"A small peptide modulates stomatal control via abscisic acid in long-distance signalling."
Nature. 556(7700):235-238 (2018) - 8.
Xiao Deng, Naoshi Dohmae, Kenneth H. Nealson, Hashimoto K, Okamoto A.:
"Multi-heme cytochromes provide a pathway for survival in energy-limited environments."
Sci Adv. 4(2):eaao5682 (2018) - 9.
Ferry L, Fournier A, Tsusaka T, Adelmant G, Shimazu T, Matano S, Kirsh O, Amouroux R, Dohmae N, Suzuki T, Filion GJ, Deng W, de Dieuleveult M, Fritsch L, Kudithipudi S, Jeltsch A, Leonhardt H, Hajkova P, Marto JA, Arita K, Shinkai Y, Defossez PA.:
"Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation."
Mol Cell. 67(4):550-65 (2017) - 10.
Shihoya W, Nishizawa T, Okuta A, Tani K, Dohmae N, Fujiyoshi Y, Nureki O, Doi T.:
"Activation mechanism of endothelin ETB receptor by endothelin-1."
Nature. 537(7620):363-68 (2016)
Recent Research Results
Related Links
Lab Members
Principal investigator
- Naoshi Dohmae
- Unit Leader
Core members
- Makoto Muroi
- Senior Research Scientist
- Hiroshi Nakayama
- Senior Research Scientist
- Makoto Kawatani
- Senior Research Scientist
- Takehiro Suzuki
- Senior Technical Scientist
Contact Information
Bioscience Building,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan
Email: dohmae [at] riken.jp