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RIKEN Center for Biosystems Dynamics Research Laboratory for Animal Resources and Genetic Engineering

Team Leader: Hiroshi Kiyonari (Ph.D.)

Research Summary

Hiroshi  Kiyonari(Ph.D.)

Our major mission is to support a wide variety of needs in laboratory animal experiments at RIKEN Kobe Campus by providing animal housing services, technical assistance and technology development in genetics and reproductive biology. Additionally, we provide education and training of personnel to ensure proper conduct of animal experiments. In recent years, we are developing new laboratory animal models, such as Gekko, Suncus and Opossum. With the expertise in advanced developmental engineering and reproductive technology, our unit closely collaborates with Genetic Engineering Team to generate and distribute genetically engineered mice for research labs in RIKEN institutes and centers, as well as those in a larger domestic and international biomedical research communities.

Main Research Fields

  • Molecular Biology & Genetics

Related Research Fields

  • Biology & Biochemistry
  • Neuroscience & Behavior

Research Subjects

  • Advancement of technologies in reproductive biology
  • In vitro development of early mouse embryos

Selected Publications

  • 1.Nakao, H., Harada, T., Nakao, K., Kiyonari, H., Inoue, K., Furuta, Y., and Aiba, A.:
    "A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes."
    Genesis, 54(2):65-77, 2016.
  • 2.Yoshida, M., Kajikawa, E., Kurokawa, D., Tokunaga, T., Onishi, A., Yonemura, S., Kobayashi, K., Kiyonari, H., and Aizawa, S.:
    "Conserved and divergent expression patterns of markers of axial development in eutherian mammals."
    Dev. Dyn., 245(1): 67-86, 2016.
  • 3.Hara, Y., Tatsumi, K., Yoshida, M., Kajikawa, E., Kiyonari, H., and Kuraku, S.:
    "Optimizing and benchmarking de novo transcriptome sequencing: from library preparation to assembly evaluation."
    BMC Genomics, 16(1):977, 2015.
  • 4.Popov, L.M., Marceau, C.D., Starkl, P.M., Lumb, J.H., Shah, J., Guerrera, D. Cooper, R.L., Merakou. C., Bouley. D.M., Meng, W., Kiyonari, H., Takeichi, M., Galli, S.J., Bagnoli, F., Citi, S., Carette, J.E., and Amieva, M.R.:
    "The adherens junctions control susceptibility to Staphylococcus aureus α-toxin."
    Proc. Natl. Acad. Sci. U S A, 112(46): 14337-14342, 2015.
  • 5.Hirate, Y., Hirahara, S., Inoue, K., Kiyonari, H., Niwa, H., and Sasaki, H.:
    "Par-aPKC-dependent and -independent mechanisms cooperatively control cell polarity, Hippo signaling, and cell positioning in 16-cell stage mouse embryos."
    Dev. Growth Differ., 57(8): 544-556, 2015.
  • 6.Katayama, A., Nakatsuka, A., Eguchi, J., Murakami, K., Teshigawara, S., Kanzaki, M., Nunoue, T., Hida, K., Wada, N., Yasunaka, T., Ikeda, F., Takaki, A., Yamamoto, K., Kiyonari, H., Makino, H., and Wada, J.:
    "Beneficial impact of Gpnmb and its significance as a biomarker in nonalcoholic steatohepatitis."
    Sci. Rep., 5:16920, 2015.
  • 7.Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, CL., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., and Takahashi, R.:
    "The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway."
    PLoS Genet., 11(9): e1005503, 2015.
  • 8.Nakatsu, Y., Iwashita, M., Sakoda, H., Ono, H., Nagata, K., Matsunaga, Y., Fukushima, T., Fujishiro, M., Kushiyama, A., Kamata, H., Takahashi, S., Katagiri, H., Honda, H., Kiyonari, H., Uchida, T., and Asano, T.:
    "Prolyl isomerase Pin1 negatively regulates AMP-activated protein kinase (AMPK) by associating with the CBS domain in the γ subunit."
    J. Biol. Chem., 290(40): 24255-24266, 2015.
  • 9.Kurimoto, K., Yabuta, Y., Hayashi, K., Ohta, H., Kiyonari, H., Mitani, T., Moritoki, Y., Kohri, K., Kimura, H., Yamamoto, T., Katou, Y., Shirahige, K., and Saitou, M.:
    "Quantitative Dynamics of Chromatin Remodeling during Germ Cell Specification from Mouse Embryonic Stem Cells."
    Cell Stem Cell. 16(5): 517-532, 2015.
  • 10.Lee, M.S., Hwang, K.S., Oh, H.W., Ji-Ae, K., Kim, H.T., Cho, H.S., Lee, J.J., Yeong Ko, J., Choi, J.H., Jeong, Y.M., You, K.H., Kim, J., Park, D.S., Nam, K.H., Aizawa, S., Kiyonari, H., Shioi, G., Park, J.H., Zhou, W., Kim, N.S., and Kim, C.H.:
    "IFT46 plays an essential role in cilia development."
    Dev. Biol. 400(2): 248-257, 2015.

Recent Research Results

Related Links

Lab Members

Principal investigator

Hiroshi Kiyonari
Team Leader

Core members

Takaya Abe
Technical Scientist
Kenichi Inoue
Expert Technician
Mayo Shigeta
Expert Technician
Mari Kaneko
Technical Staff
Miho Sato
Technical Staff
Aki Shiraishi
Technical Staff
Tomoko Tokunaga
Technical Staff
Tomoko Tokuhara
Technical Staff
Arisa Tobe
Technical Staff
Kohei Nakano
Technical Staff
Miwako Nomura
Technical Staff
Kana Bando
Technical Staff
Michiko Higashikawa
Technical Staff
Daisuke Yamamoto
Technical Staff
Riko Yoshimi
Technical Staff
Megumi Watase
Technical Staff
Masayo Okugawa

Contact Information

2-2-3 Minatojima-minamimachi, Chuo-ku
Kobe, Hyogo
650-0047 Japan
Email: hkiyo [at] cdb.riken.jp