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RIKEN Center for Biosystems Dynamics Research Laboratory for Human Organogenesis

Team Leader: Minoru Takasato (Ph.D.)

Research Summary

Minoru  Takasato(Ph.D.)

By precisely recapitulating the developmental process of human kidney in directed differentiation of human pluripotent stem cells, we are trying to achieve the ultimate goal, generation of a three-dimensional urinary tract including the kidney and the bladder that is functional and transplantable to the patients. We also study about how to recapitulate the development of other organs that connect to the kidney. We are highly interested in studies of human embryology. Utilizing our unique technology that generates hPSCs-derived kidney organoids from the pluripotent stage in vitro, we are especially investigating developmental mechanisms of human mesoderm and urinary organs.

Main Research Fields

  • Biology

Related Research Fields

  • Medicine, Dentistry & Pharmacy

Keywords

  • Kidney development
  • iPS cell
  • Directed differentiation
  • Kidney organoid
  • three-dimensional organogenesis

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1. Susaki, E. A. & Takasato, M.
    "Perspective: Extending the Utility of Three-Dimensional Organoids by Tissue Clearing Technologies."
    Front. Cell Dev. Biol. 9, 1–12 (2021)
  • 2. Tomoda, K., Hu, H., Sahara, Y., Sanyal, H., Takasato, M. & Kime, C.
    "Reprogramming epiblast stem cells into pre-implantation blastocyst cell-like cells."
    Stem Cell Reports 16, 1197–1209 (2021)
  • 3. Knarston, I. M., Pachernegg, S., Robevska, G., Ghobrial, I., Er, P. X., Georges, E., Takasato, M., et al.
    "An In Vitro Differentiation Protocol for Human Embryonic Bipotential Gonad and Testis Cell Development."
    Stem Cell Reports 15, 1377–1391 (2020)
  • 4. Takasato, M. & Wymeersch, F. J.
    "Challenges to future regenerative applications using kidney organoids."
    Curr. Opin. Biomed. Eng. 13, 144–151 (2020)
  • 5. Phipson, B., Er, P. X., Combes, A. N., Forbes, T. A., Howden, S. E., Zappia, L., Yen, H.-J., Lawlor, K. T., Hale, L. J., Sun, J., Wolvetang, E., Takasato, M., Oshlack, A. & Little, M. H.
    "Evaluation of variability in human kidney organoids."
    Nat. Methods 16, 79–87 (2019)
  • 6. Takasato, M.
    "Advice for the Next Generation: Minoru Takasato."
    Cell Stem Cell 24, 688–689 (2019).
  • 7. *Takasato, M., Er, P. X., Chiu, H. S. & Little, M. H.
    "Generation of kidney organoids from human pluripotent stem cells."
    Nat. Protoc. 11, 1681–92 (2016)
  • 8. *Takasato, M., Er, P. X., Chiu, H. S., Maier, B., Baillie, G. J., Ferguson, C., Parton, R. G., Wolvetang, E. J., Roost, M. S., Chuva de Sousa Lopes, S. M. & Little, M. H.
    "Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis."
    Nature 526, 564–8 (2015)
  • 9. *Takasato, M. & Little, M. H.
    "The origin of the mammalian kidney: implications for recreating the kidney in vitro."
    Development 142, 1937–1947 (2015)
  • 10. *Takasato, M., Er, P. X., Becroft, M., Vanslambrouck, J. M., Stanley, E. G., Elefanty, A. G. & Little, M. H.
    "Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney."
    Nat. Cell Biol. 16, 118–126 (2014)

Lab Members

Principal investigator

Minoru Takasato
Team Leader

Core members

Wei Zhao
Research Scientist
Filip Jos Wymeersch
Research Scientist
Junichi Taniguchi
Special Postdoctoral Researcher
Olena Trush
Research Scientist
Yoshiki Sahara
Visiting Scientist
Chie Fukui
Technical Staff I
Kensuke Yabuuchi
Research Associate
Kazuhiro Ofuji
Research Associate
Rio Noto
Student Trainee
Wataru Uno
Junior Research Associate
Yukari Usuda
Student Trainee
Taiki Satoh
Junior Research Associate
Masaya Goto
Student Trainee
Eri Yamashita
Administrative Part-time Worker I

Contact Information

3F, RIKEN BDR Developmental Biology Bldg.A,
2-2-3 Minatojima-minamimachi, Chuou-ku,
Kobe, Hyogo,
650-0047 Japan
Email: minoru.takasato [at] riken.jp

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