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RIKEN Center for Biosystems Dynamics Research Laboratory for Protein Functional and Structural Biology

Team Leader: Mikako Shirouzu (Ph.D.)

Research Summary

Mikako  Shirouzu(Ph.D.)

The high-resolution structural information of proteins related to diseases shall increasingly become important for drug development leading to future individualized medicine. We plan to establish a structural analysis technology platform to contribute to "Life innovation" such as drug discovery as well as development of methods for the sample preparation of challenging proteins including membrane proteins/biomolecular complexes and for the structural analysis by cryo-electron microscopy (cryo-EM). The 3D-structural information will be used for in-silico screening/design of chemical compounds and for dynamic structural analysis toward simulation research of cell function.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Medicine, Dentistry & Pharmacy

Keywords

  • Protein preparation
  • Cryo-electron microscopy
  • X-ray crystallography
  • Drug discovery

Selected Publications

  • 1.Kujirai, T., Ehara, H., Fujino, Y., et al.:
    “Structural basis of the nucleosome transition during RNA polymerase II passage”
    Science 362(6414), pp.595-598 (2018)
  • 2.Shigematsu, H., Imasaki, T., Doki, C., et al.:
    “Structural insight into microtubule stabilization and kinesin inhibition by Tau-family MAPs”
    The Journal of Cell Biology 217(12), pp.4155-4163 (2018)
  • 3.Shima, T., Morikawa, M., Kaneshiro, J., et al.:
    “Kinesin-binding–triggered conformation switching of microtubules contributes to polarized transport”
    The Journal of Cell Biology 217(12), pp.4164-4183 (2018)
  • 4.Ohbayashi, N., Murayama, K., Kato-Murayama, M., et al.:
    “Structural Basis for the Inhibition of Cyclin G-Associated Kinase by Gefitinib“
    ChemistryOpen 7(9), 721-727, (2018)
  • 5.Kasuya, G., Nakane, T., Yokoyama, T., et al.:
    “Cryo-EM structures of the human volume-regulated anion channel LRRC8”
    Nature Structural & Molecular Biology 25(9), pp.797-804 (2018)
  • 6.Yamagata A, Miyazaki Y, Yokoi N, et al.:
    “Structural basis of epilepsy-related ligand–receptor complex LGI1–ADAM22”
    Nature communications 9(1), pp.1546 (2018)
  • 7.Matsuda T, Ito T, Takemoto C, et al.:
    “Cell-free synthesis of functional antibody fragments to provide a structural basis for antibody-antigen interaction”
    PLoS One 13(2), pp.e0193158 (2018)
  • 8.Hosaka T, Okazaki M, Kimura-Someya T, et al.:
    “Crystal structural characterization reveals novel oligomeric interactions of human voltage-dependent anion channel 1”
    Protein Science 26(9), pp.1749-1758 (2017)
  • 9.Shinoda T, Shinya N, Ito K, et al.:
    “Structural basis for disruption of claudin assembly in tight junctions by an enterotoxin”
    Scientific Reports 6, pp.33632 (2016)
  • 10.Masuda M, Uno Y, Ohbayashi N, et al.:
    “TNIK inhibition abrogates colorectal cancer stemness”
    Nature Communications 7, pp.2586 (2016)

Publications

Related Links

Lab Members

Principal investigator

Mikako Shirouzu
Team Leader

Contact Information

C316 3F Central Research Building,
1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa
230-0045, Japan
Tel: +81-(0)45-503-9202
Fax: +81-(0)45-503-9264

Email: mikako.shirouzu [at] riken.jp

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