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RIKEN Center for Biosystems Dynamics Research Laboratory for Protein Functional and Structural Biology

Team Leader: Mikako Shirouzu (Ph.D.)

Research Summary

Mikako  Shirouzu(Ph.D.)

The high-resolution structural information of proteins related to diseases shall increasingly become important for drug development leading to future individualized medicine. We plan to establish a structural analysis technology platform to contribute to "Life innovation" such as drug discovery as well as development of methods for the sample preparation of challenging proteins including membrane proteins/biomolecular complexes and for the structural analysis by cryo-electron microscopy (cryo-EM). The 3D-structural information will be used for in-silico screening/design of chemical compounds and for dynamic structural analysis toward simulation research of cell function.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Medicine, Dentistry & Pharmacy

Keywords

  • Protein preparation
  • Cryo-electron microscopy
  • X-ray crystallography
  • Drug discovery

Selected Publications

  • 1. Needham, D. M., Yoshizawa, S., Hosaka, T., et al.:
    "A distinct lineage of giant viruses brings a rhodopsin photosystem to unicellular marine predators"
    Proceedings of the National Academy of Sciences of the United States of America, 116(41), pp.20574-20583 (2019)
  • 2. Lee, Y., Wiriyasermkul, P., Jin, C., Quan, L., et al.:
    "Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc"
    Nature Structural & Molecular Biology 26(6), pp.510-517. (2019)
  • 3. Yokoyama, T., Machida, K., Iwasaki, W., et al.:
    "HCV IRES Captures an Actively Translating 80S Ribosome"
    Molecular Cell 74(6), pp.1205-1214 (2019).
  • 4. Kashiwagi, K., Yokoyama, T., Nishimoto, M., et al.:
    "Structural basis for eIF2B inhibition in integrated stress response"
    Science 364(6439), pp.495-499 (2019)
  • 5. Ehara, H., Kujirai, T., Fujino, Y., Shirouzu, M., et al.:
    "Structural insight into nucleosome transcription by RNA polymerase II with elongation factors"
    Science 363(6428), pp.744-747 (2019)
  • 6. Kujirai, T., Ehara, H., Fujino, Y., et al.:
    "Structural basis of the nucleosome transition during RNA polymerase II passage"
    Science 362(6414), pp.595-598 (2018)
  • 7. Shigematsu, H., Imasaki, T., Doki, C., et al.:
    "Structural insight into microtubule stabilization and kinesin inhibition by Tau-family MAPs"
    The Journal of Cell Biology 217(12), pp.4155-4163 (2018)
  • 8. Shima, T., Morikawa, M., Kaneshiro, J., et al.:
    "Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport"
    The Journal of Cell Biology 217(12), pp.4164-4183 (2018)
  • 9. Yamagata, A., Miyazaki, Y., Yokoi, N., et al.:
    "Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22"
    Nature communications 9(1), pp.1546 (2018)
  • 10. Matsuda, T., Ito, T., Takemoto, C., et al.:
    "Cell-free synthesis of functional antibody fragments to provide a structural basis for antibody-antigen interaction"
    PLoS One 13(2), pp.e0193158 (2018)

Publications

Related Links

Lab Members

Principal investigator

Mikako Shirouzu
Team Leader

Contact Information

C316 3F Central Research Building,
1-7-22 Suehiro-cho,
Tsurumi-ku, Yokohama, Kanagawa
230-0045, Japan
Tel: +81-(0)45-503-9202
Fax: +81-(0)45-503-9264

Visit

Email: mikako.shirouzu [at] riken.jp

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