Research Summary

Selected Publications

• 1.Ehara, H., Kujirai, T., Fujino, Y., Shirouzu, M., Kurumizaka, H., and Sekine, S.:
  “Structural insight into nucleosome transcription by RNA polymerase II with elongation factors.”
  Science 363(6428), 744-747. (2019)
• 2.Kujirai, T., Ehara, H., Fujino, Y., Shirouzu, M., Sekine, S., and Kurumizaka, H.;
  “Structural basis of the nucleosome transition during RNA polymerase II passage.”
  Science 362(6414), 595-598. (2018)
• 3.Ooi, WY., Murayama, Y., Mekler, V., Minakhin, L., Severinov, K., Yokoyama, S., and Sekine, S.:
  “A Thermus phage protein inhibits host RNA polymerase by preventing template DNA strand loading during open promoter complex formation.”
  Nucleic Acids Research 46(1), 431-441. (2018)
• 4.Ehara, H., Yokoyama, T., Shigematsu, H., Yokoyama, S., Shirouzu, M., and Sekine, S.:
  “Structure of the complete elongation complex of RNA polymerase II with basal factors.”
  Science 357(6354), 921-924. (2017)
• 5.Shimizu, H., Miyazaki, H., Ohsawa, N., Shoji, S., Ishizuka-Katsura, Y., Tosaki, A., Oyama, F., Terada, T., Sakamoto, K., Shirouzu, M., Sekine, S., Nukina, N., and Yokoyama, S.:
  “Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits.”
  Scientific Reports 6, 26618. (2016)
• 6.Sekine, S., Murayama, Y., Svetlov, V., Nudler, E., and Yokoyama, S.:
  “The ratcheted and ratchetable structural states of RNA polymerase underlie multiple transcriptional functions.”
  Molecular Cell 57(3), 408-421. (2015)
• 7.Naganuma, M., Sekine, S., Chong, Y.E., Guo, M., Yang, X.L., Gamper, H., Hou, Y.M., Schimmel, P., and Yokoyama, S.:
  “The selective tRNA aminoacylation mechanism based on a single G·U pair.”
  Nature 510(7506), 507-511. (2014)
• 8.Tagami, S., Sekine, S., Minakhin, L., Esyunina, D., Akasaka, R., Shirouzu, M., Kulbachinskiy, A., Severinov, K., and Yokoyama, S.:
  “Structural basis for promoter specificity switching of RNA polymerase by a phage factor.”
  Genes & Development 28(5), 521-531. (2014)
• 9.Itoh, Y, Brocker, MJ, Sekine, S, Hammond, G., Suetsugu, S., Sol,l D., and Yokoyama, S.:
  “Decameric SelA·tRNASec Ring Structure Reveals Mechanism of Bacterial Selenocysteine Formation.”
  Science 340(6128), 75-78. (2013)
• 10.Tagami, S., Sekine, S., Kumarevel, T., Hino, N., Murayama, Y., Kamegamori, S., Yamamoto, M., Sakamoto, K., and Yokoyama, S.:
  “Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein.”
  Nature 468(7326), 978-982. (2010)

Recent Research Results

• 

  Oct. 31, 2023
  Atomic picture of dengue replication could transform antiviral approaches

• 

  Nov. 17, 2022
  Gene-reading enzyme razes and rebuilds DNA-winding structures in its path

• 

  Apr. 12, 2019
  Elongation factors smooth transcription in the nucleosome

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