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RIKEN Center for Advanced Photonics Live Cell Super-Resolution Imaging Reseach Team

Team Leader: Akihiko Nakano (D.Sci.)

Research Summary

Akihiko  Nakano(D.Sci.)

Light is a cutting-edge tool for life science research. Development of useful fluorescent probes and the advancement of light microscope technologies have brought us a new world of “live” imaging within a cell. We are developing super-resolution confocal live imaging microscopy (SCLIM) by the combination of a high-speed confocal scanner and a high-sensitivity camera system. With this method we will observe membrane trafficking and organellar dynamics in living cells at high-speed and super-resolution (4D) and elucidate underlining molecular mechanisms. We will also try to extend this technology to medical and pharmacological applications.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Complex Systems
  • Interdisciplinary Science & Engineering
  • Biology

Keywords

  • membrane traffic
  • vesicular transport
  • super-resolution live imaging
  • confocal microscopy
  • organelles

Selected Publications

  • 1. Shimizu, Y., Takagi, J., Ito, E., Ito, Y., Ebine, K., Komatsu, Y., Goto, Y., Sato, M., Toyooka, K., Ueda, T., Kurokawa, K., Uemura, T., and Nakano, A.:
    "Cargo sorting zones in the trans-Golgi network visualized by super-resolution confocal live imaging microscopy in plants"
    Nat. Commun. 12, 1901 (2021).
  • 2. Rizzo, R., Russo, D., Kurokawa, K., Nakano, A., Corda, D., D’Angelo, G., Luini, A. et al.:
    "Golgi maturation-dependent glycoenzyme recycling controls glycosphingolipid biosynthesis and cell growth via GOLPH3"
    EMBO J. 40, e107238 (2021).
  • 3. Rodriguez-Gallardo, S., Kurokawa, K., Sabido-Bozo, S., Cortes-Gomez, A., Ikeda, A., Zoni, V., Aguilera-Romero, A., Perez-Linero, A.M., Lopez, S., Waga, M., Araki, M., Nakano, M., Riezman, H., Funato, K., Vanni, S., Nakano, A., and Muñiz, M.:
    "Ceramide chain length-dependent protein sorting into selective endoplasmic reticulum exit sites"
    Sci. Adv. 6, eaba8237 (2020).
  • 4. Tojima, T., Suda, Y., Ishii, M., Kurokawa, K., and Nakano, A.:
    "Spatiotemporal dissection of the trans-Golgi network in budding yeast"
    J. Cell Sci. 132, jcs231159 (2019).
  • 5. Kurokawa, K., Osakada, H., Kojidani, T., Suda, Y., Asakawa, H., Haraguchi, T., and Nakano, A.:
    "Visualization of secretory cargo transport within the Golgi apparatus in living yeast cells"
    J. Cell Biol. 218, 1602-1618 (2019).
  • 6. Uemura, T., Nakano, R.T., Takagi, J., Wang, Y., Kramer, K., Finkemeier, I., Nakagami, H., Tsuda, K., Ueda, T., Schulze-Lefert, P., and Nakano, A.:
    "A Golgi-released subpopulation of the trans-Golgi network mediates protein secretion in Arabidopsis"
    Plant Physiol. 179, 519-532 (2019).
  • 7. Ito, Y., Uemura, T., and Nakano, A.:
    "Golgi Entry Core Compartment functions as the COPII-independent scaffold for ER-Golgi transport in plant cells"
    J. Cell Sci. 131, jcs203893 (2018).
  • 8. Ito, Y,. Toyooka, K., Fujimoto, M., Ueda, T., Uemura, T., and Nakano, A.:
    "The trans-Golgi network and the Golgi stacks behave independently during regeneration after Brefeldin A treatment in tobacco BY-2 cells"
    Plant Cell Physiol. 58, 811-821 (2017).
  • 9. Ishii, M., Suda, Y., Kurokawa, K., and Nakano, A.:
    "COPI is essential for Golgi cisternal maturation and dynamics"
    J. Cell Sci. 129, 3251-3261 (2016).
  • 10. Kurokawa, K., Suda, Y., and Nakano, A.:
    "Sar1 localizes at the rims of COPII-coated membranes in vivo"
    J. Cell Sci. 129, 3231-3237 (2016).

Recent Research Results

Related Links

Lab Members

Principal investigator

Akihiko Nakano
Team Leader

Core members

Kazuo Kurokawa
Senior Research Scientist
Takuro Tojima
Research Scientist
Daisuke Miyashiro
Postdoctoral Researcher
Tomohiro Uemura
Visiting Scientist
Yasuyuki Suda
Visiting Scientist
Yoko Ito
Visiting Scientist
Yasuhito Kosugi
Technical Staff I
Kumiko Ishii
Technical Staff II

Contact Information

S411 Bioscience Building,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan
Email: nakano [at] riken.jp

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