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RIKEN Center for Advanced Photonics Live Cell Super-Resolution Imaging Reseach Team

Team Leader: Akihiko Nakano (D.Sci.)

Research Summary

Akihiko  Nakano(D.Sci.)

Light is a cutting-edge tool for life science research. Development of useful fluorescent probes and the advancement of light microscope technologies have brought us a new world of “live” imaging within a cell. We are developing super-resolution confocal live imaging microscopy (SCLIM) by the combination of a high-speed confocal scanner and a high-sensitivity camera system. With this method we will observe membrane trafficking and organellar dynamics in living cells at high-speed and super-resolution (4D) and elucidate underlining molecular mechanisms. We will also try to extend this technology to medical and pharmacological applications.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Complex Systems
  • Interdisciplinary Science & Engineering
  • Biology


  • membrane traffic
  • vesicular transport
  • super-resolution live imaging
  • confocal microscopy
  • organelles

Selected Publications

  • 1.Yoko Ito, Kiminori Toyooka, Masaru Fujimoto, Takashi Ueda, Tomohiro Uemura, and Akihiko Nakano:
    "The trans-Golgi network and the Golgi stacks behave independently during regeneration after Brefeldin A treatment in tobacco BY-2 cells"
    Plant Cell Physiol. 58:811-821 (2017).
  • 2.Midori Ishii, Yasuyuki Suda, Kazuo Kurokawa, and Akihiko Nakano:
    "COPI is essential for Golgi cisternal maturation and dynamics"
    J. Cell Sci. 129:3251-3261 (2016).
  • 3.Kazuo Kurokawa, Yasuyuku Suda and Akihiko Nakano:
    "Sar1 localizes at the rims of COPII-coated membranes in vivo"
    J. Cell Sci. 129:3231-3237 (2016).
  • 4.Masakazu Iwai, Makio Yokono, Kazuo Kurokawa, Akira Ichihara, and Akihiko Nakano:
    "Live-cell visualization of excitation energy dynamics in chloroplast thylakoid structures"
    Sci. Rep. 6:29940 (2016).
  • 5.Kazuo Kurokawa, Michiyo Okamoto, and Akihiko Nakano:
    "Contact of cis-Golgi with ER exit sites executes cargo capture and delivery from the ER"
    Nat. Commun. 5:3653 (2014).
  • 6.Tomohiro Uemura, Yasuyuki Suda, Takashi Ueda, and Akihiko Nakano:
    "Dynamic behavior of the trans-Golgi network in root tissues of Arabidopsis revealed by super-resolution live imaging"
    Plant Cell Physiol. 55:694-670 (2014).
  • 7.Ryogo Hirata, Coh-ichi Nihei, and Akihiko Nakano:
    "Isoform-selective oligomer formation of Saccharomyces cerevisiae p24 family proteins"
    J. Biol. Chem. 288:37057-37070 (2013).
  • 8.Yasuyuki Suda, Kazuo Kurokawa, Ryogo Hirata, and Akihiko Nakano:
    "Rab GAP cascade regulates dynamics of Ypt6 during the Golgi maturation"
    Proc. Natl. Acad. Sci. U. S. A. 110:18976-18981 (2013).
  • 9.Yoko Ito, Tomohiro Uemura, Keiko Shoda, Masaru Fujimoto, Takashi Ueda, and Akihiko Nakano:
    "cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells"
    Mol. Biol. Cell 23:3203-3214 (2012).
  • 10.Michiyo Okamoto, Kazuo Kurokawa, Kumi Matsuura-Tokita, Chieko Saito, Ryogo Hirata, and Akihiko Nakano:
    "High-curvature domains of the endoplasmic reticulum (ER) are important for the organization of ER exit sites in Saccharomyces cerevisiae"
    J. Cell Sci. 125:3412-3420 (2012).

Recent Research Results

Related Links

Lab Members

Principal investigator

Akihiko Nakano
Team Leader

Core members

Kazuo Kurokawa
Senior Research Scientist
Takuro Tojima
Research Scientist
Daisuke Miyashiro
Postdoctoral Researcher
Tomohiro Uemura
Visiting Scientist
Yasuyuki Suda
Visiting Scientist
Yoko Ito
Visiting Scientist
Yasuhito Kosugi
Technical Staff I
Kumiko Ishii
Technical Staff II

Contact Information

S411 Bioscience Building,
2-1 Hirosawa,
Wako, Saitama
351-0198, Japan
Email: nakano [at] riken.jp