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RIKEN Center for Biosystems Dynamics Research Laboratory for Advanced Biomolecular Engineering

Team Leader: Shunsuke Tagami (Ph.D.)

Research Summary

Shunsuke  Tagami(Ph.D.)

This team aims to engineer functional bio-macromolecules applicable to medical and basic biological researches, by integrating technologies in synthetic and structural biology including in vitro reconstruction of target protein complexes, in vitro evolution of protein/RNA, X-ray crystallography, and structure based protein engineering.


  • Protein evolution
  • Protein structure
  • Functional peptides

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1. Sumida T, Dubiley S, Wilcox B, Severinov K, Tagami S.:
    "Structural Basis of Leader Peptide Recognition in Lasso Peptide Biosynthesis Pathway"
    ACS Chem. Biol., 14(7), 1619-1627 (2019)
  • 2. *Tagami S, Attwater J, Holliger P.:
    "Simple peptides derived from the ribosomal core potentiate RNA polymerase ribozyme function"
    Nature Chemistry, 9, 325-332 (2017).
  • 3. Tagami S, Sekine S, Minakhin L, Esyunina D, Akasaka R, Shirouzu M, Kulbachinskiy A, Severinov K, Yokoyama S.:
    "Structural basis for promoter specificity switching of RNA polymerase by a phage factor"
    Genes Dev, 28(5), 521-531 (2014)
  • 4. Tagami S, Sekine S, Kumarevel T, Hino N, Murayama Y, Kamegamori S, Yamamoto M, Sakamoto K, Yokoyama S.:
    "Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein."
    Nature, 468(7326), 978-982 (2010)

Recent Research Results

Related Links

Lab Members

Principal investigator

Shunsuke Tagami
Team Leader

Core members

Akira Wada
Senior Research Scientist
Almasul Alfi
Research Scientist
Peiying Li
Research Scientist
Kazumasa Ohtake
Visiting Scientist
Sota Yagi
Visiting Scientist
Aleksandr Popov
Junior Research Associate
Takuto Furuhashi
Student Trainee
Tami Kosuge

Contact Information

Room C516, Central Building,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Tel: +81-(0)45-503-9205