RIKEN BioResource Research Center (BRC^®) Integrative Developmental Engineering Division

Division Director: Kimiko Inoue (Ph.D.)

Japanese Page

Research Summary

Our life begins with fertilization and progresses through changes such as implantation, birth, growth, and aging, and this cycle is passed on to the next generation through the germ cells. This sequence of processes is called the “life cycle”.
In our laboratory, we aim to elucidate various life phenomena in the life cycle by using developmental engineering technology with oocytes, sperm, and embryos, as well as genetic and epigenetic analysis technology. Furthermore, based on the knowledge obtained from these studies, we will contribute to the establishment of more advanced developmental engineering technologies for the maintenance and supply of high-quality and reliable bioresources.

Main Research Fields

Biology

Related Research Fields

Agricultural Sciences
Biological Sciences
Medicine, Dentistry & Pharmacy
Animal life science-related
Laboratory animal science-related
Genome biology-related

Keywords

Developmental Engineering
Life cycle
Epigenetics
Germ cells
Experimental animals

Selected Publications

1. Hirose, M., Inoue, K., Matoba, S., Tatebe, T., Tokita, S., Dodo, Y., Tomishima, T., Hasegawa, A., Honda, A., Ozaki, M., Shinogi, A., Yanagisawa, R., Fauzi, M., Murakami, T., Inagaki, N., Tamura, M., and Ogura, A.
"Disruption of insulin receptor substrate 2 (IRS2) causes non-obese type 2 diabetes with β-cell dysfunction in the golden (Syrian) hamster."
Sci Rep, 14: 17450. (2024)
2. Guo, Y., Kitano, T., Inoue, K., Murano, K., Hirose, M., Li, TD., Sakashita, A., Ishizu, H., Ogonuki, N., Matoba, S., Sato, M., Ogura, A., and Siomi, H.
"Obox4 promotes zygotic genome activation upon loss of Dux."
eLife, 13:e95856. (2024)
3. Watanabe, N., Hirose, M., Hasegawa, A., Mochida, K., Ogura, A., and Inoue, K.
"Derivation of embryonic stem cells from wild-derived mouse strains by nuclear transfer using peripheral blood cells."
Sci Rep, 13: 11175. (2023)
4. Ogonuki, N., Kyogoku, H., Hino, T., Osawa, Y., Fujiwara, Y., Inoue, K., Kunieda, T., Mizuno, S., Tateno, H., Sugiyama, F., Kitajima, T., and Ogura, A.
"Birth of mice from meiotically arrested spermatocytes following biparental meiosis in halved oocytes."
EMBO Rep, 23: e54992. (2022)
5. Inoue, K.
Mouse somatic cell nuclear transfer: What has changed and remained unchanged in 25 years.
J. Reprod. Dev., 69, 129–138. (2023)
6. Hada, M., Miura, H., Tanigawa, A., Matoba, S., Inoue, K., Ogonuki, N., Hirose, M., Watanabe, N., Nakato, R., Fujiki, K., Hasegawa, A., Sakashita, A., Okae, H., Miura, K., Shikata, D., Arima, T., Shirahige, K., Hiratani, I., and Ogura, A.
"Highly rigid H3.1/H3.2-H3K9me3 domains set a barrier for cell fate reprogramming in trophoblast stem cells."
Genes Dev., 36, 84-102. (2022)
7. Inoue, K., Ogonuki, N., Kamimura, S., Inoue, H., Matoba, S., Hirose, M., Honda, A., Miura, K., Hada, M., Hasegawa, A., Watanabe, N., Dodo, Y., Mochida, K., and Ogura, A.
"Loss of H3K27me3 imprinting in the Sfmbt2 miRNA cluster causes enlargement of cloned mouse placentas."
Nat. Commun., 11, 2150. (2020)
8. Inoue, K., Hirose, M., Inoue, H., Hatanaka, Y., Honda, A., Hasegawa, A., Mochida, K., and Ogura, A.
"The rodent-specific microRNA cluster within the Sfmbt2 gene is imprinted and essential for placental development."
Cell Rep., 19, 949-956. (2017)
9. Matoba, S., Inoue, K., Kohda, K., Sugimioto, M., Mizutani, E., Ogonuki, N., Nakamura, T., Abe, K., Nakano, T., Ishino, F., and Ogura, A.
"RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos."
Proc. Natl. Acad. Sci. USA, 108, 20621-20626. (2011)
10. Inoue, K., Kohda, T., Sugimoto, M., Sado, T., Ogonuki, N., Matoba, S., Shiura, H., Ikeda, R., Mochida, K., Fujii, T., Sawai, K., Otte, AP., Tian, XC., Yang, X., Ishino, F., Abe, K., and Ogura, A.
"Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer."
Science 330, 496-499. (2010)