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RIKEN Center for Integrative Medical Sciences Proteome Homeostasis Research Unit

Unit Leader: Koshi Imami (Ph.D.)

Research Summary

Koshi Imami

Our unit aims to elucidate how the protein homeostasis (“proteostasis”) is regulated and maintained on a proteome-wide scale. In particular, we will focus on the dynamics of nascent proteins to understand how the cellular proteome is shaped at the level of translation. To this end, we develop cutting-edge proteomic technologies to capture protein-level information, including translation, degradation, post-translational modifications, activity, and protein-protein interactions. We will contribute to biomedical sciences by elucidating cellular homeostasis and disease mechanisms at the protein level.

Main Research Fields

  • Biological Sciences

Related Research Fields

  • Chemistry
  • Complex Systems
  • Biological Sciences
  • Biology
  • Medicine, Dentistry & Pharmacy
  • System genome science-related
  • Genome biology-related
  • Chemical biology-related


  • Proteomics
  • Mass spectrometry
  • Translation
  • Post- or co-translational modification
  • Systems biology

Selected Publications

Papers with an asterisk(*) are based on research conducted outside of RIKEN.

  • 1. Tsumagari K, Isobe Y, Ishihama Y, Seita J, Arita M, Imami K.
    "Application of liquid-liquid extraction for N-terminal myristoylation proteomics"
    Molecular & Cellular Proteomics, 100677 (2023)
  • 2. Li Y, Kanao E, Yamano T, Ishihama Y, Imami K.
    "TurboID-EV: Proteomic Mapping of Recipient Cellular Proteins Proximal to Small Extracellular Vesicles"
    Analytical Chemistry, 95(38):14159-14164 (2023)
  • 3. *Imami K, Selbach M, Ishihama Y.
    "Monitoring mitochondrial translation by pulse SILAC"
    Journal of Biological Chemistry, 299:102865 (2023)
  • 4. *Harnett D, Ambrozkiewicz MC, Zinnall U, Rusanova A, Borisova E, Drescher AN, Couce-Iglesias M, Villamil G, Dannenberg R, Imami K, Münster-Wandowski A, Fauler B, Mielke T, Selbach M, Landthaler M, Spahn CMT, Tarabykin V, Ohler U, Kraushar ML.
    "A critical period of translational control during brain development at codon resolution"
    Nature Structural & Molecular Biology, 12:1277-1290 (2022)
  • 5. *Uchiyama J, Roy R, Wang DO, Morikawa K, Kawahara Y, Iwasaki M, Yoshino C, Mishima Y, Ishihama Y, Imami K.
    "pSNAP: Proteome-wide analysis of elongating nascent polypeptide chains"
    iScience, 25:104516 (2022)
  • 6. *Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Günnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Münster-Wandowski A, Bürger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT.
    "Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit"
    Molecular Cell, 81:304-322 (2021)
  • 7. *Uchiyama J, Ishihama Y, Imami K.
    "Quantitative nascent proteome profiling by dual-pulse labelling with O-propargyl-puromycin and stable isotope-labelled amino acids"
    Journal of Biochemistry, 169:227-236 (2021)
  • 8. *Theil K, Imami K, Rajewsky N.
    "Identification of proteins and miRNAs that specifically bind an mRNA in vivo"
    Nature Communications, 10:4205 (2019)
  • 9. *Imami K, Milek M, Bogdanow B, Yasuda T, Kastelic N, Zauber H, Ishihama Y, Landthaler M, Selbach M.
    "Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis"
    Molecular Cell, 72:84-98 (2018)
  • 10. *Wessels HH(#), Imami K(# co-first), Baltz AG, Kolinski M, Beldovskaya A, Selbach M, Small S, Ohler U, Landthaler M.
    "The mRNA-bound proteome of the early fly embryo"
    Genome Research, 26:1000-9 (2016)

Recent Research Results

Lab Members

Principal investigator

Koshi Imami
Unit Leader

Core members

Kazuya Tsumagari
Special Postdoctoral Researcher
Phebee Angeline Devadasan Racheal
Postdoctoral Researcher
Qingshun Lin
Technical Staff I
Kanako Igarashi
Research Part-time Worker I

Contact Information

North Research Building,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Email: koshi.imami [at] riken.jp