RIKEN Center for Integrative Medical Sciences Proteome Homeostasis Research Unit
Unit Leader: Koshi Imami (Ph.D.)
Research Summary

Our unit is dedicated to uncovering the mechanisms that maintain protein homeostasis (“proteostasis”) on a proteome-wide scale. Specifically, we focus on the dynamics of nascent proteins to understand how the cellular proteome is shaped during translation. To achieve this, we are developing advanced proteomic technologies to comprehensively capture protein-level insights, including translation, degradation, post-translational modifications, activity, and protein-protein interactions. By unraveling the intricacies of cellular homeostasis and disease mechanisms at the proteome level, we aim to make significant contributions to biomedical sciences.
Main Research Fields
- Biological Sciences
Related Research Fields
- Chemistry
- Complex Systems
- Biological Sciences
- Biology
- Medicine, Dentistry & Pharmacy
- System genome science-related
- Genome biology-related
- Chemical biology-related
Keywords
- Proteomics
- Mass spectrometry
- Translation
- Post- or co-translational modification
- Systems biology
Selected Publications
Papers with an asterisk(*) are based on research conducted outside of RIKEN.
- 1.
Tsumagari K, Isobe Y, Ishihama Y, Seita J, Arita M, Imami K.
"Application of liquid-liquid extraction for N-terminal myristoylation proteomics"
Molecular & Cellular Proteomics, 100677 (2023) - 2.
Li Y, Kanao E, Yamano T, Ishihama Y, Imami K.
"TurboID-EV: Proteomic Mapping of Recipient Cellular Proteins Proximal to Small Extracellular Vesicles"
Analytical Chemistry, 95(38):14159-14164 (2023) - 3.
*Imami K, Selbach M, Ishihama Y.
"Monitoring mitochondrial translation by pulse SILAC"
Journal of Biological Chemistry, 299:102865 (2023) - 4.
*Harnett D, Ambrozkiewicz MC, Zinnall U, Rusanova A, Borisova E, Drescher AN, Couce-Iglesias M, Villamil G, Dannenberg R, Imami K, Münster-Wandowski A, Fauler B, Mielke T, Selbach M, Landthaler M, Spahn CMT, Tarabykin V, Ohler U, Kraushar ML.
"A critical period of translational control during brain development at codon resolution"
Nature Structural & Molecular Biology, 12:1277-1290 (2022) - 5.
*Uchiyama J, Roy R, Wang DO, Morikawa K, Kawahara Y, Iwasaki M, Yoshino C, Mishima Y, Ishihama Y, Imami K.
"pSNAP: Proteome-wide analysis of elongating nascent polypeptide chains"
iScience, 25:104516 (2022) - 6.
*Kraushar ML, Krupp F, Harnett D, Turko P, Ambrozkiewicz MC, Sprink T, Imami K, Günnigmann M, Zinnall U, Vieira-Vieira CH, Schaub T, Münster-Wandowski A, Bürger J, Borisova E, Yamamoto H, Rasin MR, Ohler U, Beule D, Mielke T, Tarabykin V, Landthaler M, Kramer G, Vida I, Selbach M, Spahn CMT.
"Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit"
Molecular Cell, 81:304-322 (2021) - 7.
*Uchiyama J, Ishihama Y, Imami K.
"Quantitative nascent proteome profiling by dual-pulse labelling with O-propargyl-puromycin and stable isotope-labelled amino acids"
Journal of Biochemistry, 169:227-236 (2021) - 8.
*Theil K, Imami K, Rajewsky N.
"Identification of proteins and miRNAs that specifically bind an mRNA in vivo"
Nature Communications, 10:4205 (2019) - 9.
*Imami K, Milek M, Bogdanow B, Yasuda T, Kastelic N, Zauber H, Ishihama Y, Landthaler M, Selbach M.
"Phosphorylation of the Ribosomal Protein RPL12/uL11 Affects Translation during Mitosis"
Molecular Cell, 72:84-98 (2018) - 10.
*Wessels HH(#), Imami K(# co-first), Baltz AG, Kolinski M, Beldovskaya A, Selbach M, Small S, Ohler U, Landthaler M.
"The mRNA-bound proteome of the early fly embryo"
Genome Research, 26:1000-9 (2016)
Recent Research Results
Jan. 19, 2024
Hot on the tracks of extracellular vesicles
Lab Members
Principal investigator
- Koshi Imami
- Unit Leader
Core members
- Kazuya Tsumagari
- Special Postdoctoral Researcher
- Phebee Angeline Devadasan Racheal
- Postdoctoral Researcher
- Qingshun Lin
- Technical Staff I
- Kanako Igarashi
- Research Part-time Worker I
- Kaho Takamuro
- Student Trainee
Contact Information
North Research Building,
1-7-22 Suehiro-cho, Tsurumi-ku,
Yokohama City, Kanagawa,
230-0045, Japan
Email: koshi.imami@riken.jp