RIKEN Center for Sustainable Resource Science Cell Factory Research Team

Team Leader: Akihiko Kondo (Ph.D.)

Japanese Page

Research Summary

The Cell Factory Research Team organized in fiscal year 2012 develops optimum technologies for the construction of microorganisms producing biomonomers, a material in chemical products, from plant materials. We have also reinforced our collaboration with the industrial world in order to develop technologies to produce those biomonomers from plant materials such as herbaceous and woody biomasses.

Main Research Fields

Engineering

Related Research Fields

Agricultural Sciences
Informatics
Biology
Biotechnology
Bioinformatics

Keywords

Synthetic biology
Metabolic engineering
Bioproduction
Metabolic design
Enzyme engineering

Selected Publications

1. Mitsui, R., Kondo, A, Shirai, T.:
"Production of (R)-citramalate by engineered Saccharomyces cerevisiae."
Metabolic Engineering Communications, 19, e00247. (2024).
2. Noda, S., Mori, Y., Ogawa, Y., Fujiwara, R., Dainin, M., Shirai, T., Kondo, A.:
"Metabolic and enzymatic engineering approach for the production of 2-phenylethanol in engineered Escherichia coli."
Bioresource Technology, 406, 130927. (2024).
3. Vikromvarasiri, N., Noda, S., Shirai, T., Kondo, A.:
"Investigation of two metabolic engineering approaches for (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis."
Journal Biological Engineering, 17, 3. (2023).
4. Tanaka, K., Shirai, T., Vavricka, C.J., Matsuda, M., Kondo, A., Hasunuma, T.:
"Dark accumulation of downstream glycolytic intermediates initiates robust photosynthesis in cyanobacteria"
Plant Physiology. 191, 2400-2413. (2023).
5. Shirai, T., Kondo, A.:
"In Silico Design Strategies for the Production of Target Chemical Compounds Using Iterative Single-Level Linear Programming Problems"
Biomolecules, 12, 620. (2022).
6. Vikromvarasiri, N., Shirai, T., Kondo, A.:
"Metabolic engineering design to enhance (R,R)-2,3-butanediol production from glycerol in Bacillus subtilis based on flux balance analysis"
Microbial Cell Factory. 20, 196. (2021).
7. Noda, S., Mori, Y., Fujiwara, R., Shirai, T., Tanaka, T., Kondo, A.
"Reprogramming Escherichia coli pyruvate-forming reaction towards chorismate derivatives production"
Metabolic Engineering 67, 1-10. (2021).
8. Mori, Y., Noda, S., Shirai, T., Kondo, A.:
"Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant"
Nature Communications, 12, 2195. (2021).
9. Noda, S., Shirai, T., Mori, Y., Oyama, S., Kondo, A.:
"Engineering a synthetic pathway for maleate in Escherichia coli"
Nature Communications. 8, 1153. (2017).
10. Shirai, T., Osanai, T., Kondo, A.:
"Designing intracellular metabolism for production of target compounds by introducing a heterologous metabolic reaction based on a Synechosystis sp. 6803 genome-scale model"
Microbial Cell Factory, 15, 13. (2016).