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RIKEN SPring-8 Center Life Science Research Infrastructure Group

Group Director: Masaki Yamamoto (Ph.D.)

Research Summary

Masaki  Yamamoto(Ph.D.)

Macromolecular crystallography (MX) of difficult target such as membrane proteins has been successfully achieved by using bright light source of SPring-8. To emphasize the application of structural analysis such as better understanding of life and drug discovery, our group advance the research facilities (SPring-8 and SACLA) for MX or small angle X-ray scattering (Bio-SAXS). Moreover, we facilitate the use of electron cryo-microscopy (cryo-EM) to expect synergy on structural biology activity in addition to those techniques using X-ray. Our group tackle with new structural life science for complex structure-function analysis by combining such state-of-the-art analytical techniques.

Main Research Fields

  • Biology

Related Research Fields

  • Chemistry
  • Engineering
  • Biological Sciences
  • Agricultural Sciences
  • Medicine, Dentistry & Pharmacy
  • Structural biochemistry
  • Biophysics
  • Drug development chemistry

Keywords

  • Structural life Science
  • Structural Biology
  • Correlative Structural Analysis
  • Drug Discovery
  • Structural Dynamics

Selected Publications

  • 1.Hideki Shigematsu, Tsuyoshi Imasaki, Chihiro Doki, Takuya Sumi, Mari Aoki, Tomomi Uchikubo-Kamo, Ayako Sakamoto, Kiyotaka Tokuraku, Mikako Shirouzu, and Ryo Nitta.:
    “Structural insight into microtubule stabilization and kinesin inhibition by Tau family MAPs”
    Journal of Cell Biology 217 (12), 4155?4163 (2018)
  • 2.Masaki Yamamoto, Kunio Hirata, Keitaro Yamashita, Kazuya Hasegawa, Go Ueno, Hideo Ago, Takashi Kumasaka.:
    “Protein microcrystallography using synchrotron radiation”
    IUCrJ 4 (5), 529-539 (2017)
  • 3.M Suga, F Akita, M Sugahara, M Kubo, Y Nakajima, T Nakane, K Yamashita, Y Umena, M Nakabayashi, T Yamane, T Nakano, M Suzuk, T Masuda, S Inoue, T Kimura, T Nomura, S Yonekura, L-J Yu, T Sakamoto, T Motomura, J-H Chen, Y Kato1, T Noguchi, K Tono, Y Joti, T Kameshima, T Hatsui, E Nango, R Tanaka, H Naitow, Y Matsuura, A Yamashita, M Yamamoto, O Nureki, M Yabashi, T Ishikawa, S Iwata, J-R Shen.:
    “Light-induced structural changes and the site of O=O bond formation in PSII
    caught by XFEL”
    Nature 543(7643):131-135 (2017)
  • 4.Shigeyuki Matsumoto, Nao Miyano, Seiki Baba, Jingling Liao, Takashi Kawamura, Chiemi Tsuda, AzusaTakeda A, Masaki Yamamoto, Takashi Kumasaka, Toru Kataoka, Fumi Shima.:
    “Molecular Mechanism for Conformational Dynamics of Ras?GTP Elucidated from In-Situ Structural Transition in Crystal”
    Scientific Report 6, 25931 (2016)
  • 5.Deng, D, Sun, PC, Yan, CY, Ke, M, Jiang, X, Xiong, L, Ren, WL, Hirata, K, Yamamoto, M, Fan, SL and Yan, N.:
    “Molecular basis of ligand recognition and transport by glucose transporters”
    Nature 526 (7573), 391-396.(2015)
  • 6.Tanabe, H, Fujii, Y, Okada-Iwabu, M, Iwabu, M, Nakamura, Y, Hosaka, T, Motoyama, K, Ikeda, M, Wakiyama, M, Terada, T, Ohsawa, N, Hato, M, Ogasawara, S, Hino, T, Murata, T, Iwata, S, Hirata, K, Kawano, Y, Yamamoto, M, Kimura-Someya, T, Shirouzu, M, Yamauchi, T, Kadowaki, T, and Yokoyama,S.:
    “Crystal structures of the human adiponectin receptors”
    Nature 520 (7547), 312-316 (2015)
  • 7.Shima, F, Yoshikawa, Y., Ye, M., Araki, M, Matsumoto, S., Liao, JL., Hu, LZ., Sugimoto, T ., Ijiri, Y., Takeda, A., Nishiyama, Y., Sato, C., Muraoka, S.,Tamura, A., Osoda, T., Tsuda, K., Miyakawa, T., Fukunishi, H., Shimada, J., Kumasaka, T., Yamamoto, M., Kataoka, T.:
    “In silico discovery of small-molecule Ras inhibitors that display antitumor activity by blocking the Ras-effector interaction”
    Proc. Nat. Acad. Sci the USA 110, 8182-8187 (2013)
  • 8.Janet L Smith, Robert F Fischetti and Masaki Yamamoto.:
    “Micro-crystallography comes of age”
    Current Opinion in Structural Biology 22, 602?612 (2012)

Related Links

Lab Members

Principal investigator

Masaki Yamamoto
Group Director

Contact Information

Structural Biology Facility,
1-1-1 Kouto, Sayo-cho,
Sayo-gun, Hyogo,
679-5148 Japan
Tel: +81-(0)791-58-2839
Fax: +81-(0)791-58-2834
Email: yamamoto [at] riken.jp

Organization

SR Life Science Instrumentation Team
Masaki Yamamoto (Ph.D.)

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