Research Summary

Selected Publications

• 1.Keitaro Yamashita, Kunio Hirata, Masaki Yamamoto.:
  "KAMO: towards automated data processing for microcrystals."
  Acta Crystallographica Section D 74 (5), 441-449 (2018)
• 2.Kazuya Hasegawa, Keitaro Yamashita, T Murai, Nipawan Nuemket, Kunio Hirata, Go Ueno, Hideo Ago, Toru Nakatsu, Takashi Kumasaka, Masaki Yamamoto.:
  ”Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography”
  Journal of Synchrotron Radiation 24 (1), 29-41 (2017)
• 3.Amane Kobayashi, Yuki Sekiguchi, Yuki Takayama, Tomotaka Oroguchi, Keiya Shirahama, Yasufumi Torizuka Masahiro Manoda, Masayoshi Nakasako, and Masaki Yamamoto.:
  "TAKASAGO-6 apparatus for cryogenic coherent X-ray diffraction imaging of biological non-crystalline particles using X-ray free electron laser at SACLA."
  Review of Scientific Instruments 87, 053109 (2016)
• 4.Keitaro Yamashita, Dongqing Pan, Tomohiko Okuda, Michihiro Sugahara, Atsushi Kodan, Tomohiro Yamaguchi, Tomohiro Murai, Keiko Gomi, Naoki Kajiyama, Eiichi Mizohata, Mamoru Suzuki, Eriko Nango, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Jaehyun Park, Changyong Song, Takaki Hatsui, Makina Yabashi, So Iwata, Hiroaki Kato, Hideo Ago, Masaki Yamamoto, and Toru Nakatsu.:
  "An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography."
  Scientific Reports 5、 14017 (2015)
• 5.Suga, M., Akita, F., Hirata, K., Ueno, G., Murakami, H., Nakajima, Y., Shimizu, T., Yamashita, K., Yamamoto, M., Ago, H., and Shen J.R.:
  “Native structure of photosystem II at 1.95A resolution viewed by femtosecond X-ray pulses”
  Nature 517, 99-103 (2015).
• 6.Hirata, K., Shinzawa-Itoh, K., Yano, N., Takemura, S., Kato, K., Hatanaka, M., Muramoto, K., Kawahara, T., Tsukihara, T., Yamashita, E., Tono, K., Ueno, G., Hikima, T., Murakami, H., Inubushi, Y., Yabashi, M., Ishikawa, T., Yamamoto, M., Ogura, T., Sugimoto, H., Shen, J. R., *Yoshikawa, S. and Ago, H.:
  ”Determination of damage-free crystal structure of an X-ray-sensitive protein using an XFEL”
  Nature Methods 11, 734-746 (2014)
• 7.Kumazaki, K., Chiba, S., Takemoto, M., Furukawa, A., Nishiyama, K., Sugano, Y., Mori, T., Dohmae, N., Hirata, K., Nakada-Nakura, Y., Maturana, AD., Tanaka, Y., Mori, H., Sugita, Y., Arisaka, F., Ito, K., Ishitani, R., Tsukazaki, T., Nureki, O.:
  "Structural basis of Sec-independent membrane protein insertion by YidC"
  Nature 509, 516?520 (2014)
• 8.Nishizawa, T., Kita, S., Maturana, A.D., Furuya, N., Hirata, K., Kasuya, G., Ogasawara, S., Dohmae, N., Iwamoto, T., Ishitani, R., Nureki, O.:
  "Structural Basis for the Counter-Transport Mechanism of a H+/Ca2+ Exchanger."
  Science 341, (6142): 168-172 (2013)
• 9.Nakasako, M, Takayama, Y, Oroguchi, T, Sekiguchi Y, Kobayashi, A, Shirahama, K, Yamamoto, M, Hikima, T, Yonekura, K, Maki-Yonekura, S, Kohmura Y, Inubushi, Y, Takahashi, Y, Suzuki A, Matsunaga, S, Inui, Y, Tono, K, Kameshima, T, Joti, Y, Hoshi, T.:
  "KOTOBUKI-1 apparatus for cryogenic coherent X-ray diffraction imaging"
  Rev. Sci. Instrum. 84, 093705 (2013).
• 10.Hirata, K., Kawano, Y., Ueno, G., Hashimoto, K., Murakami, H., Hasegawa, K., Hikima, T., Kumasaka, T., Yamamoto, M.:
  "Achievement of protein micro-crystallography at SPring-8 beamline BL32XU."
  J. Phys.: Conf. Ser. 425, 012002 (2013).

Recent Research Results

• 

  Feb. 9, 2018
  A light touch for enzyme analysis

• 

  Dec. 15, 2017
  How bacteria avoid poisoning themselves

• 

  Sep. 22, 2017
  Watching enzymes inhale

• 

  Feb. 6, 2015
  Shining a gentle light on photosynthesis

• Jul. 18, 2014
  X-ray studies without the damage

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