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Chief Scientist Laboratories Biofunctional Synthetic Chemistry Laboratory

Chief Scientist: Katsunori Tanaka (Ph.D.)

Research Summary

Katsunori  Tanaka(Ph.D.)

Although many efficient bond-forming reactions have been developed in the field of synthetic organic chemistry, their utility typically fails to translate in complex mixtures present in cellular or living biosystems. As such, only a handful of effective labeling or conjugation methods in biosystems are readily available. To address this issue, we are synthetically exploring the unique reactivity of conjugated imines for their potential in novel chemical reactions. The benefit of this approach is that since a large part of amine-containing biomacromolecules are biosynthesized through imine chemistry, imine-mediated chemical reactions will not face the same challenges that classical synthetic organic chemical reactions face within living systems. In parallel to this work, our studies have also shown that the unique reactivity of imines could be involved in pathways that regulate biologically important processes.

Besides usage in methods of labeling or conjugation, the novel reactivity of imines, in combination with other advanced metal-catalyzed transformations, could alternatively be used in the multi-step synthesis of biofunctional molecules within living biosystems. Through a concept we refer to as “Therapeutic In Vivo Synthetic Chemistry”, we aim to develop an adaptable system where a cascade of organic transformations can be directly executed at target regions within the body during predefined times to generate a bioactive molecule that elicits a localized biological effect. Towards this goal, we are analyzing, with the use of molecular imaging, the complex “pattern recognition” mechanisms of natural glycans in vivo and applying the glycan-based interactions to direct various linked biomolecules to desired organs and tissues.

Main Research Fields

  • Chemistry

Related Research Fields

  • Complex Systems
  • Biological Sciences
  • Biology
  • Medicine, Dentistry & Pharmacy
  • Biomolecular Chemistry
  • Chemical Biology
  • Biofunctional Chemistry

Keywords

  • Molecular Imaging
  • Theranostics
  • Bioactive Compound
  • Glycan
  • Therapeutic In Vivo Synthetic Chemistry

Selected Publications

  • 1. A. R. Pradipta, T. Tanei, K. Morimoto, K. Shimazu, S. Noguchi and K. Tanaka.:
    "Emerging technologies for real-time intraoperative margin assessment in future breast-conserving surgery"
    Adv. Sci., 7 (9), 1901519 (2020).
  • 2. K. Vong, S. Eda, Y. Kadota, I. Nasibullin, T. Wakatake, S. Yokoshima, K. Shirasu and K. Tanaka.:
    "An artificial metalloenzyme biosensor can detect ethylene gas in fruits and Arabidopsis leaves"
    Nature Commun., 10, 5746 (2019).
  • 3. Y. Manabe, R. Marchetti, Y. Takakura, M. Nagasaki, W. Nihei, T. Takebe, K. Tanaka, K. Kabayama, F. Chiodo, S. Hanashima, Y. Kamada, E. Miyoshi, H. P. Dulal, Y. Yamaguchi, Y. Adachi, N. Ohno, H. Tanaka, A. Silipo, K. Fukase and A. Molinaro.:
    "The core fucose on an IgG antibody is an endogenous ligand of Dectin-1"
    Angew. Chem. Int. Ed., 58, 18697-18702 (2019).
  • 4. S. Eda, I. Nasibullin, K. Vong, N. Kudo, M. Yoshida, A. Kurbangalieva and K. Tanaka.:
    "Biocompatibility and therapeutic potential of glycosilated albumin artificial metalloenzymes"
    Nature Catal., 2, 780-792 (2019).
  • 5. K. Fujiki, Y. Kanayama, S. Yano, N. Sato, T. Yokokita, P. Ahmadi, Y. Watanabe, H. Haba and K. Tanaka.:
    "211At-Labeled immunoconjugate via a one-pot three-component double click strategy: "Practical access to α-emission cancer radiotherapeutics"
    Chem. Sci., 10, 1936-1944 (2019).
  • 6. T. Tanei, A. R. Pradipta, K. Morimoto, M. Fujii, M. Arata, A. Ito, M. Yoshida, E. Saigitbatalova, A. Kurbangalieva, J.-I. Ikeda, E. Morii, S. Noguchi and K. Tanaka.:
    "Cascade reaction in human live tissue allows clinically applicable diagnosis of breast cancer morphology"
    Adv. Sci., 6, 1801479 (2019).
  • 7. M. Taichi, S. Nomura, I. Nakase, R. Imamaki, Y. Kizuka, F. Ota, N. Dohmae, S. Kitazume, N. Taniguchi, and K. Tanaka.:
    "In-situ ligation of high- and low-affinity ligands to cell surface receptors enables highly selective recognition"
    Adv. Sci., 1700147 (2017).
  • 8. K. Tsubokura, K. K. H. Vong, A. R. Pradipta, A. Ogura, S. Urano, T. Tahara, S. Nozaki, H. Onoe, Y. Nakao, R. Sibgatullina, A. Kurbangalieva, Y. Watanabe and K. Tanaka.:
    "In vivo gold complex catalysis within live mice"
    Angew. Chem. Int. Ed., 56, 3579-3584 (2017).
  • 9. L. Latypova, R. Sibgatullina, A. Ogura, K. Fujiki, A. Khabibrakhmanova, T. Tahara, S. Nozaki, S. Urano, K, Tsubokura, H. Onoe, Y. Watanabe, A. Kurbangalieva and K. Tanaka.:
    "Sequential double "clicks" toward structurally well-defined heterogeneous N-glycoclusters: The importance of cluster heterogeneity on pattern recognition in vivo"
    Adv. Sci., 4, 1600394 (2017).
  • 10. A. Tsutsui, T. Zako, T. Bu, Y. Yamaguchi, M. Maeda and K. Tanaka.:
    "1,5-Diazacyclooctanes, as Exclusive Oxidative Polyamine Metabolites, Inhibit Amyloid-β(1-40) Fibrillization"
    Adv. Sci., 3, 1600082 (2016).

Recent Research Results

Annual research report

Related Links

Lab Members

Principal investigator

Katsunori Tanaka
Chief Scientist

Core members

Akihiro Ishiwata
Senior Research Scientist
Kenward Vong
Research Scientist
Shogo Nomura
Postdoctoral Researcher
Peni Ahmadi
Postdoctoral Researcher
Igor Nasibullin
Postdoctoral Researcher
Tsung-che Chang
Postdoctoral Researcher
Tomoya Yamamoto
Postdoctoral Researcher
Sayaka Urano
Technical Staff II
Yixuan Lin
International Program Associate
Ivan Smirnov
Intern
Yukishige Ito
Visiting Scientist
Yu Nakagawa
Visiting Scientist
Satoru Tamura
Visiting Scientist
Koji Morimoto
Visiting Scientist
Ambara Rachmat Pradipta
Visiting Scientist
Katsumasa Fujiki
Visiting Scientist
Kyohei Muguruma
Visiting Scientist
Takefumi Murase
Visiting Scientist
Yasuko Egawa
Research Part-time Worker II
Yuriko Kusakari
Research Part-time Worker II
Masayo Ohara
Assistant

Contact Information

N508-1 Chemistry and Materials Physics Building,
2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Email: kotzenori [at] riken.jp

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